Determination and Comparision of Micelle Radius of Different Surfactants Using Förster Resonance Energy Transfer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F17%3APU124318" target="_blank" >RIV/00216305:26310/17:PU124318 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination and Comparision of Micelle Radius of Different Surfactants Using Förster Resonance Energy Transfer

Popis výsledku v původním jazyce

This contribution is focused on utilization of Förster resonance energy transfer as a nanoscale ruler in order to determine micellar radii of three cationic surfactants with different hydrophobic chain length – CTAB (C16), Septonex (C15) and TTAB (C14). As a convenient donor-acceptor pair, Perylene (which is solubilized inside micelles) and Fluorescein (situated in the outer hydrophilic part of micelles) were chosen. Located in close proximity (nm), Fluorescein quenches fluorescence of Perylene, the effectivity of which is in direct relation to their distance – radius of micelle in this case. Results obtained by FRET were compared with FCS (Fluorescence Correlation Spectroscopy) measurements, which determine micelle radius on the basis of its diffusion coefficient. In this experiment micelles were labeled only with Perylene in order to avoid effects of fluorescent labeling on the surface of micelles.

Název v anglickém jazyce

Determination and Comparision of Micelle Radius of Different Surfactants Using Förster Resonance Energy Transfer

Popis výsledku anglicky

This contribution is focused on utilization of Förster resonance energy transfer as a nanoscale ruler in order to determine micellar radii of three cationic surfactants with different hydrophobic chain length – CTAB (C16), Septonex (C15) and TTAB (C14). As a convenient donor-acceptor pair, Perylene (which is solubilized inside micelles) and Fluorescein (situated in the outer hydrophilic part of micelles) were chosen. Located in close proximity (nm), Fluorescein quenches fluorescence of Perylene, the effectivity of which is in direct relation to their distance – radius of micelle in this case. Results obtained by FRET were compared with FCS (Fluorescence Correlation Spectroscopy) measurements, which determine micelle radius on the basis of its diffusion coefficient. In this experiment micelles were labeled only with Perylene in order to avoid effects of fluorescent labeling on the surface of micelles.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10403 - Physical chemistry

Projekt

<a href="/cs/project/LO1211" target="_blank" >LO1211: Centrum materiálového výzkumu na FCH VUT v Brně - udržitelnost a rozvoj</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů