Hyaluronan interactions with cationic surfactants-Insights from fluorescence resonance energy transfer and anisotropy techniques
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F22%3APU144804" target="_blank" >RIV/00216305:26310/22:PU144804 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/abs/pii/S0141813022010376?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0141813022010376?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2022.05.067" target="_blank" >10.1016/j.ijbiomac.2022.05.067</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Hyaluronan interactions with cationic surfactants-Insights from fluorescence resonance energy transfer and anisotropy techniques
Popis výsledku v původním jazyce
Interactions of hyaluronan with micelles formed by cationic surfactants were studied by the time-resolved measurement of fluorescence resonance energy transfer (FRET) using perylene and fluorescein as probes. Two surfactants were studied - Cetyltrimethylammonium bromide (CTAB) and Septonex. In pure micellar solutions, the same values of FRET efficiency were found for both surfactants, but values for the binding of the probe pair were lower for Septonex micelles than in the case of CTAB. This was attributed to steric effects of the carbethoxy group in the Septonex polar head. Upon the addition of hyaluronan, decreased FRET efficiency and increased binding were detected in comparison with pure surfactants. To resolve the structure of the formed aggregates, steady state and time-resolved fluorescence anisotropy was employed as an additional technique. All results indicated that cationic micelles bind to hyaluronan forming a pearl necklace structure with micelles of smaller size compared to pure surfactant. Besides theoretical interest, the studied polyelectrolyte-surfactant system may be interesting for the formulation of drug delivery systems.
Název v anglickém jazyce
Hyaluronan interactions with cationic surfactants-Insights from fluorescence resonance energy transfer and anisotropy techniques
Popis výsledku anglicky
Interactions of hyaluronan with micelles formed by cationic surfactants were studied by the time-resolved measurement of fluorescence resonance energy transfer (FRET) using perylene and fluorescein as probes. Two surfactants were studied - Cetyltrimethylammonium bromide (CTAB) and Septonex. In pure micellar solutions, the same values of FRET efficiency were found for both surfactants, but values for the binding of the probe pair were lower for Septonex micelles than in the case of CTAB. This was attributed to steric effects of the carbethoxy group in the Septonex polar head. Upon the addition of hyaluronan, decreased FRET efficiency and increased binding were detected in comparison with pure surfactants. To resolve the structure of the formed aggregates, steady state and time-resolved fluorescence anisotropy was employed as an additional technique. All results indicated that cationic micelles bind to hyaluronan forming a pearl necklace structure with micelles of smaller size compared to pure surfactant. Besides theoretical interest, the studied polyelectrolyte-surfactant system may be interesting for the formulation of drug delivery systems.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10403 - Physical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/GC19-14024J" target="_blank" >GC19-14024J: Hyaluronan-kataniontové vezikulární komplexy - interakce, struktura, fyzikální vlastnosti a stabilita</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN
0141-8130
e-ISSN
1879-0003
Svazek periodika
211
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
9
Strana od-do
107-115
Kód UT WoS článku
000806366000001
EID výsledku v databázi Scopus
2-s2.0-85129961353