THE EFFECT OF CO-ENCAPSULATION OF PROBIOTICS WITH PREBIOTICS ON THE VIABILITY OF PROBIOTIC BACTERIA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F17%3APU136129" target="_blank" >RIV/00216305:26310/17:PU136129 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

THE EFFECT OF CO-ENCAPSULATION OF PROBIOTICS WITH PREBIOTICS ON THE VIABILITY OF PROBIOTIC BACTERIA

Popis výsledku v původním jazyce

The aim of this work was to evaluate the effect of four natural prebiotics (inulin, psyllium, apple fiber and hemp fiber) on viability of probiotic bacteria. Two probiotic strains, namely Lactobacillus acidophilus (CCM 4833) and Bifidobacterium breve (CCM 7825T) were co-encapsulated with selected prebiotics into alginate particles. The viability of encapsulated microorganisms during storage in acidic conditions was analysed. Also, the physicochemical evaluation of prepared particles and the viability of cells during simulated gastrointestinal conditions were tested. To analysis of probiotics light/fluorescence microscopy and flow cytometry were used. Results indicated significant improvement in survival of co-encapsulated cells compared to free cells. As the best prebiotic for co-encapsulation the hemp fiber was found. The hemp fiber exhibited the highest increase of cells count and the high viability of encapsulated bacterial cells during long-term storage. The co-encapsulation of probiotics into alginate particles leads to increased tolerance of bacteria to acidic environment. Particles also maintained their integrity during passage through the gastrointestinal tract until they reached their target destination. Therefore, prepared particles could be used in foods or food supplements with targeted transport of probiotic bacteria.

Název v anglickém jazyce

THE EFFECT OF CO-ENCAPSULATION OF PROBIOTICS WITH PREBIOTICS ON THE VIABILITY OF PROBIOTIC BACTERIA

Popis výsledku anglicky

The aim of this work was to evaluate the effect of four natural prebiotics (inulin, psyllium, apple fiber and hemp fiber) on viability of probiotic bacteria. Two probiotic strains, namely Lactobacillus acidophilus (CCM 4833) and Bifidobacterium breve (CCM 7825T) were co-encapsulated with selected prebiotics into alginate particles. The viability of encapsulated microorganisms during storage in acidic conditions was analysed. Also, the physicochemical evaluation of prepared particles and the viability of cells during simulated gastrointestinal conditions were tested. To analysis of probiotics light/fluorescence microscopy and flow cytometry were used. Results indicated significant improvement in survival of co-encapsulated cells compared to free cells. As the best prebiotic for co-encapsulation the hemp fiber was found. The hemp fiber exhibited the highest increase of cells count and the high viability of encapsulated bacterial cells during long-term storage. The co-encapsulation of probiotics into alginate particles leads to increased tolerance of bacteria to acidic environment. Particles also maintained their integrity during passage through the gastrointestinal tract until they reached their target destination. Therefore, prepared particles could be used in foods or food supplements with targeted transport of probiotic bacteria.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

<a href="/cs/project/LO1211" target="_blank" >LO1211: Centrum materiálového výzkumu na FCH VUT v Brně - udržitelnost a rozvoj</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2016, 8TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION

ISBN

978-80-87294-71-0

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

548-553

Název nakladatele

TANGER

Místo vydání

Ostrava, Czech republic

Místo konání akce

Brno

Datum konání akce

19. 10. 2016

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000410656100096