G-quasruplex structure in proximity of p53 target sequence causes significant inhibition on transactivation potential of p53 isoforms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F20%3APU137932" target="_blank" >RIV/00216305:26310/20:PU137932 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

G-quasruplex structure in proximity of p53 target sequence causes significant inhibition on transactivation potential of p53 isoforms

Popis výsledku v původním jazyce

p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis and senescence. The human TP53 gene contains alternative promoters that produce C-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. We evaluated the influence of G-quadruplex structure on the transactivation potential of C-terminal p53 isoforms in a panel of S. cerevisiae luciferase reporter strains. The targeting of p53 RE by the replacement of the ICORE cassette, using transfected single strand oligonucleotides, was performed following the Delitto Perfetto technique. Yeast isogenic strains differing in the p53 target site (with and without cloned G-quadruplex structure) were transformed with plasmid for the expression of p53 isoform proteins. Here we present results

Název v anglickém jazyce

G-quasruplex structure in proximity of p53 target sequence causes significant inhibition on transactivation potential of p53 isoforms

Popis výsledku anglicky

p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis and senescence. The human TP53 gene contains alternative promoters that produce C-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. We evaluated the influence of G-quadruplex structure on the transactivation potential of C-terminal p53 isoforms in a panel of S. cerevisiae luciferase reporter strains. The targeting of p53 RE by the replacement of the ICORE cassette, using transfected single strand oligonucleotides, was performed following the Delitto Perfetto technique. Yeast isogenic strains differing in the p53 target site (with and without cloned G-quadruplex structure) were transformed with plasmid for the expression of p53 isoform proteins. Here we present results

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů