Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F24%3APU154851" target="_blank" >RIV/00216305:26310/24:PU154851 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s12223-024-01212-w#citeas" target="_blank" >https://link.springer.com/article/10.1007/s12223-024-01212-w#citeas</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-024-01212-w" target="_blank" >10.1007/s12223-024-01212-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

Popis výsledku v původním jazyce

Cyanobacteria are prokaryotic organisms characterised by their complex structures and a wide range of pigments. With their ability to fix CO2, cyanobacteria are interesting for white biotechnology as cell factories to produce various high-value metabolites such as polyhydroxyalkanoates, pigments, or proteins. White biotechnology is the industrial production and processing of chemicals, materials, and energy using microorganisms. It is known that exposing cyanobacteria to low levels of stressors can induce the production of secondary metabolites. Understanding of this phenomenon, known as hormesis, can involve the strategic application of controlled stressors to enhance the production of specific metabolites. Consequently, precise measurement of cyanobacterial viability becomes crucial for process control. However, there is no established reliable and quick viability assay protocol for cyanobacteria since the task is challenging due to strong interferences of autofluorescence signals of intercellular pigments and fluorescent viability probes when flow cytometry is used. We performed the screening of selected fluorescent viability probes used frequently in bacteria viability assays. The results of our investigation demonstrated the efficacy and reliability of three widely utilised types of viability probes for the assessment of the viability of Synechocystis strains. The developed technique can be possibly utilised for the evaluation of the importance of polyhydroxyalkanoates for cyanobacterial cultures with respect to selected stressor-repeated freezing and thawing. The results indicated that the presence of polyhydroxyalkanoate granules in cyanobacterial cells could hypothetically contribute to the survival of repeated freezing and thawing.

Název v anglickém jazyce

Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

Popis výsledku anglicky

Cyanobacteria are prokaryotic organisms characterised by their complex structures and a wide range of pigments. With their ability to fix CO2, cyanobacteria are interesting for white biotechnology as cell factories to produce various high-value metabolites such as polyhydroxyalkanoates, pigments, or proteins. White biotechnology is the industrial production and processing of chemicals, materials, and energy using microorganisms. It is known that exposing cyanobacteria to low levels of stressors can induce the production of secondary metabolites. Understanding of this phenomenon, known as hormesis, can involve the strategic application of controlled stressors to enhance the production of specific metabolites. Consequently, precise measurement of cyanobacterial viability becomes crucial for process control. However, there is no established reliable and quick viability assay protocol for cyanobacteria since the task is challenging due to strong interferences of autofluorescence signals of intercellular pigments and fluorescent viability probes when flow cytometry is used. We performed the screening of selected fluorescent viability probes used frequently in bacteria viability assays. The results of our investigation demonstrated the efficacy and reliability of three widely utilised types of viability probes for the assessment of the viability of Synechocystis strains. The developed technique can be possibly utilised for the evaluation of the importance of polyhydroxyalkanoates for cyanobacterial cultures with respect to selected stressor-repeated freezing and thawing. The results indicated that the presence of polyhydroxyalkanoate granules in cyanobacterial cells could hypothetically contribute to the survival of repeated freezing and thawing.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

20800 - Environmental biotechnology

Projekt

<a href="/cs/project/GF19-29651L" target="_blank" >GF19-29651L: Ekologická role polyhydroxybutyrátu u cyanobakterií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FOLIA MICROBIOLOGICA

ISSN

0015-5632

e-ISSN

1874-9356

Svazek periodika

70

Číslo periodika v rámci svazku

jaro

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

19

Strana od-do

„“-„“

Kód UT WoS článku

001349486800001

EID výsledku v databázi Scopus

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