Biosynthesis and characterization of a novel polyhydroxyalkanoate terpolymer containing 2-methyl-4-hydroxybutyrate produced by the thermophilic bacterium Aneurinibacillus sp.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F26%3A0199416" target="_blank" >RIV/00216305:26310/26:0199416 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S014181302508599X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S014181302508599X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2025.148042" target="_blank" >10.1016/j.ijbiomac.2025.148042</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biosynthesis and characterization of a novel polyhydroxyalkanoate terpolymer containing 2-methyl-4-hydroxybutyrate produced by the thermophilic bacterium Aneurinibacillus sp.

Popis výsledku v původním jazyce

The increasing environmental burden of plastic pollution has intensified the search for sustainable alternatives, such as polyhydroxyalkanoates (PHA). In this study, we report the biosynthesis and characterization of a novel PHA terpolymer containing 2-methyl-4-hydroxybutyrate (2M4HB), produced by the thermophilic Gram-positive bacterium Aneurinibacillus sp. AFN2. Incorporation of alpha-carbon methylated monomers, such as 2M4HB, alters the thermal and material properties of PHA, resulting in more amorphous and flexible polymers compared to conventional PHA. The strain was cultivated on alpha-methyl-gamma-butyrolactone (AMGBL), either as the sole carbon source or in combination with glucose. Under optimized conditions (4 g/L AMGBL and 4 g/L glucose), the bacterium synthesized poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-2-methyl-4-hydroxybutyrate) containing up to 11 mol% of 2M4HB. The terpolymer composition was confirmed by gas chromatography and 1H nuclear magnetic resonance spectroscopy. Material characterization by size-exclusion chromatography, coupled with multi-angle light scattering detection, Fourier-transform infrared spectroscopy, and differential scanning calorimetry, revealed that incorporation of 2M4HB markedly influenced the polymer's properties. Higher 2M4HB content resulted in lower molecular weight, decreased melting temperature and enthalpy, and enhanced amorphous character. These findings highlight the exceptional metabolic versatility of Aneurinibacillus sp. AFN2, enabling direct incorporation of branched lactone-derived monomers without prior chemical hydrolysis. This work positions thermophilic members of the genus Aneurinibacillus as a promising platform for the sustainable production of PHA copolymers with tunable physicochemical properties.

Název v anglickém jazyce

Biosynthesis and characterization of a novel polyhydroxyalkanoate terpolymer containing 2-methyl-4-hydroxybutyrate produced by the thermophilic bacterium Aneurinibacillus sp.

Popis výsledku anglicky

The increasing environmental burden of plastic pollution has intensified the search for sustainable alternatives, such as polyhydroxyalkanoates (PHA). In this study, we report the biosynthesis and characterization of a novel PHA terpolymer containing 2-methyl-4-hydroxybutyrate (2M4HB), produced by the thermophilic Gram-positive bacterium Aneurinibacillus sp. AFN2. Incorporation of alpha-carbon methylated monomers, such as 2M4HB, alters the thermal and material properties of PHA, resulting in more amorphous and flexible polymers compared to conventional PHA. The strain was cultivated on alpha-methyl-gamma-butyrolactone (AMGBL), either as the sole carbon source or in combination with glucose. Under optimized conditions (4 g/L AMGBL and 4 g/L glucose), the bacterium synthesized poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-2-methyl-4-hydroxybutyrate) containing up to 11 mol% of 2M4HB. The terpolymer composition was confirmed by gas chromatography and 1H nuclear magnetic resonance spectroscopy. Material characterization by size-exclusion chromatography, coupled with multi-angle light scattering detection, Fourier-transform infrared spectroscopy, and differential scanning calorimetry, revealed that incorporation of 2M4HB markedly influenced the polymer's properties. Higher 2M4HB content resulted in lower molecular weight, decreased melting temperature and enthalpy, and enhanced amorphous character. These findings highlight the exceptional metabolic versatility of Aneurinibacillus sp. AFN2, enabling direct incorporation of branched lactone-derived monomers without prior chemical hydrolysis. This work positions thermophilic members of the genus Aneurinibacillus as a promising platform for the sustainable production of PHA copolymers with tunable physicochemical properties.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/GA22-10845S" target="_blank" >GA22-10845S: Studium role polyhydroxyalkanoátů u bakterie Schlegelella thermodepolymerans – slibného bakteriálního kandidáta pro biotechnologie nové generace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

1879-0003

Svazek periodika

—

Číslo periodika v rámci svazku

330

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

1-12

Kód UT WoS článku

001598071200015

EID výsledku v databázi Scopus

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