ASSAY OF NATURAL ANTIOXIDANTS POTENCY USING PIPETING ROBOT AND SPECTROPHOTOMETRY

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F12%3APU126850" target="_blank" >RIV/00216305:26620/12:PU126850 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

ASSAY OF NATURAL ANTIOXIDANTS POTENCY USING PIPETING ROBOT AND SPECTROPHOTOMETRY

Popis výsledku v původním jazyce

This work deals with the comparing antioxidant aktivity standards gallic acid, ferulic acid, qiercitrine and rutine with oxidative status model cell lines. The assay was carried out by methods DPPH, ABTS, DMPD, FRAP and free radicals. Antioxidant activity was determined individual standards in relation to time, concentration and temperature, compared the characteristics of each set of radicals and their antioxidant capacity. We decided to introduce fully automated assay of antioxidants using not only automated sample measurement but also processing of samples and application of reagents. Automated pipetting system epMotion 5075 and the automated spectrophotometer BS 400 were chosen for the assay purposes. All of the tested methods were found suitable for implementation in the automated assay. Owing to the capacity of the method, approximately 240 samples per hour (one sample per 15 seconds) can be assayed using the automated protocol.

Název v anglickém jazyce

ASSAY OF NATURAL ANTIOXIDANTS POTENCY USING PIPETING ROBOT AND SPECTROPHOTOMETRY

Popis výsledku anglicky

This work deals with the comparing antioxidant aktivity standards gallic acid, ferulic acid, qiercitrine and rutine with oxidative status model cell lines. The assay was carried out by methods DPPH, ABTS, DMPD, FRAP and free radicals. Antioxidant activity was determined individual standards in relation to time, concentration and temperature, compared the characteristics of each set of radicals and their antioxidant capacity. We decided to introduce fully automated assay of antioxidants using not only automated sample measurement but also processing of samples and application of reagents. Automated pipetting system epMotion 5075 and the automated spectrophotometer BS 400 were chosen for the assay purposes. All of the tested methods were found suitable for implementation in the automated assay. Owing to the capacity of the method, approximately 240 samples per hour (one sample per 15 seconds) can be assayed using the automated protocol.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/ED2.1.00%2F03.0072" target="_blank" >ED2.1.00/03.0072: Centrum senzorických, informačních a komunikačních systémů (SIX)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MENDELNET 2012

ISBN

978-80-7375-836-3

ISSN

—

e-ISSN

—

Počet stran výsledku

8

Strana od-do

1166-1173

Název nakladatele

Neuveden

Místo vydání

Neuveden

Místo konání akce

Mendel Univ, Fac Agron, Brno

Datum konání akce

21. 11. 2012

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

000366461200136