ISOLATION AND DETERMINATION OF LACTOFERRINE FROM HUMAN SALIVA USING PARAMAGNETIC PARTICLES

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F12%3APU126956" target="_blank" >RIV/00216305:26620/12:PU126956 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

ISOLATION AND DETERMINATION OF LACTOFERRINE FROM HUMAN SALIVA USING PARAMAGNETIC PARTICLES

Popis výsledku v původním jazyce

Saliva is a watery substance produced in the mouths of human and most animals. The important compound of saliva is lactoferrin. It is involved in binding and transport of metal ions such as Fe2+, Zn2+, Mn2+ and Cu2+. Because of these functions it has an antibacterial, anticancerogenic and anti-inflammatory activity. The aim of this study was to design a method based on isolation of lactoferrin from human saliva using paramagnetic particles to prepare bead-based complex with lactoferrin. This complex was subsequently determined by SFIA. Our results were correlated with ELISA using spectrophotometric detection. The limit of detection for lactoferin was 0.4 mu g.ml(-1). This method has the potential as a biosensor analysis.

Název v anglickém jazyce

ISOLATION AND DETERMINATION OF LACTOFERRINE FROM HUMAN SALIVA USING PARAMAGNETIC PARTICLES

Popis výsledku anglicky

Saliva is a watery substance produced in the mouths of human and most animals. The important compound of saliva is lactoferrin. It is involved in binding and transport of metal ions such as Fe2+, Zn2+, Mn2+ and Cu2+. Because of these functions it has an antibacterial, anticancerogenic and anti-inflammatory activity. The aim of this study was to design a method based on isolation of lactoferrin from human saliva using paramagnetic particles to prepare bead-based complex with lactoferrin. This complex was subsequently determined by SFIA. Our results were correlated with ELISA using spectrophotometric detection. The limit of detection for lactoferin was 0.4 mu g.ml(-1). This method has the potential as a biosensor analysis.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MENDELNET 2012

ISBN

978-80-7375-836-3

ISSN

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e-ISSN

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Počet stran výsledku

8

Strana od-do

1250-1257

Název nakladatele

Neuveden

Místo vydání

Neuveden

Místo konání akce

Mendel Univ, Fac Agron, Brno

Datum konání akce

21. 11. 2012

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

000366461200146