Separation of lactoferrin from human saliva using monolithic disc

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00213595" target="_blank" >RIV/62156489:43210/13:00213595 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/13:PU126741

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10337-013-2459-x" target="_blank" >http://dx.doi.org/10.1007/s10337-013-2459-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-013-2459-x" target="_blank" >10.1007/s10337-013-2459-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Separation of lactoferrin from human saliva using monolithic disc

Popis výsledku v původním jazyce

In this study, we optimized method for the isolation and detection of lactoferrin from human saliva using 3 mm short monolithic disc. We optimized the conditions for separation as flow rate 4 mL min-1 and ionic strength of effluent as 2 uM NaCl. We estimated limit of detection of whole method, which was hyphenated to the Bradford's assay, down to 100 ng mL-1. The purity of the isolated fractions was verified by sodium dodecyl sulphatepolyacrylamide gel electrophoresis and recovery of isolation was foundto be 51 % using minimally processed sample of saliva. Further, we tested the optimized method on group of healthy volunteers (n = 7). We were able to distinguish between the healthy subjects and subject suffering from celiac disease, which reported atleast 2.59 higher level of lactoferrin in comparison to healthy ones. The results were correlated with standard enzyme-linked immunosorbent assay (ELISA) kit with obtained correlation coefficient R2 = 0.8446. Analysis of lactoferrin in sa

Název v anglickém jazyce

Separation of lactoferrin from human saliva using monolithic disc

Popis výsledku anglicky

In this study, we optimized method for the isolation and detection of lactoferrin from human saliva using 3 mm short monolithic disc. We optimized the conditions for separation as flow rate 4 mL min-1 and ionic strength of effluent as 2 uM NaCl. We estimated limit of detection of whole method, which was hyphenated to the Bradford's assay, down to 100 ng mL-1. The purity of the isolated fractions was verified by sodium dodecyl sulphatepolyacrylamide gel electrophoresis and recovery of isolation was foundto be 51 % using minimally processed sample of saliva. Further, we tested the optimized method on group of healthy volunteers (n = 7). We were able to distinguish between the healthy subjects and subject suffering from celiac disease, which reported atleast 2.59 higher level of lactoferrin in comparison to healthy ones. The results were correlated with standard enzyme-linked immunosorbent assay (ELISA) kit with obtained correlation coefficient R2 = 0.8446. Analysis of lactoferrin in sa

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chromatographia

ISSN

0009-5893

e-ISSN

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Svazek periodika

76

Číslo periodika v rámci svazku

11-12

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

611-619

Kód UT WoS článku

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EID výsledku v databázi Scopus

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