A non-enzymatic sensor for sensitive determination of H2O2 using biomimetic nanocomposite

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134918" target="_blank" >RIV/00216305:26620/19:PU134918 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/19:43917131

Výsledek na webu

<a href="https://mendelnet.cz/artkey/mnt-201901-0113_A-non-enzymatic-sensor-for-sensitive-determination-of-H-sub-2-sub-O-sub-2-sub-using-biomimetic-nanocomposite.php?back=/magno/mnt/2019/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201901-0113_A-non-enzymatic-sensor-for-sensitive-determination-of-H-sub-2-sub-O-sub-2-sub-using-biomimetic-nanocomposite.php?back=/magno/mnt/2019/mn1.php?secid=4</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A non-enzymatic sensor for sensitive determination of H2O2 using biomimetic nanocomposite

Popis výsledku v původním jazyce

Hydrogen peroxide (H2O2), as a critical secondary messenger, is important for in vitro and in vivo study. In addition, it is of great significance in monitoring the biological condition under chemical oxidative stress. A non-enzymatic sensor was developed for the detection and monitoring of H2O2 using a nanocomposite (NC). The NC is prepared and modified by using multi-walled carbon nanotubes and tris(2,2’-bipyridyl) copper(II) dichloride complex acts as a mediator. The electrochemical techniques like cyclic voltammetry and chrono-amperometry were used to study the electrochemical behaviour of H2O2 at the developed electrode. The reduction peak of H2O2 shifts to less negative potential with increasing the reducing current at the developed electrode. The calibration curve of the proposed sensor was plotted using chrono-amperometry at −0.1 V as applied potential with stepwise addition of H2O2. The developed sensor has high stability and able to detect H2O2 with a high sensitivity and repeatability. The linear range was found to be 2.4–33.0 µg/ml. Furthermore, the limit of detection and the limit of quantification were 0.7 µg/ml and 2.4 µg/ml, respectively. The reproducibility of the prepared sensor was evaluated by calculating the RSD% = 7.2.

Název v anglickém jazyce

A non-enzymatic sensor for sensitive determination of H2O2 using biomimetic nanocomposite

Popis výsledku anglicky

Hydrogen peroxide (H2O2), as a critical secondary messenger, is important for in vitro and in vivo study. In addition, it is of great significance in monitoring the biological condition under chemical oxidative stress. A non-enzymatic sensor was developed for the detection and monitoring of H2O2 using a nanocomposite (NC). The NC is prepared and modified by using multi-walled carbon nanotubes and tris(2,2’-bipyridyl) copper(II) dichloride complex acts as a mediator. The electrochemical techniques like cyclic voltammetry and chrono-amperometry were used to study the electrochemical behaviour of H2O2 at the developed electrode. The reduction peak of H2O2 shifts to less negative potential with increasing the reducing current at the developed electrode. The calibration curve of the proposed sensor was plotted using chrono-amperometry at −0.1 V as applied potential with stepwise addition of H2O2. The developed sensor has high stability and able to detect H2O2 with a high sensitivity and repeatability. The linear range was found to be 2.4–33.0 µg/ml. Furthermore, the limit of detection and the limit of quantification were 0.7 µg/ml and 2.4 µg/ml, respectively. The reproducibility of the prepared sensor was evaluated by calculating the RSD% = 7.2.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2019 Proceedings of 26th International PhD Students Conference

ISBN

978-80-7509-688-3

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

585-590

Název nakladatele

Mendel University in Brno

Místo vydání

Neuveden

Místo konání akce

Brno

Datum konání akce

6. 11. 2019

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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