Heat Transfer Time Determination Based on DNA Melting Curve Analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F20%3APU132821" target="_blank" >RIV/00216305:26620/20:PU132821 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s10404-019-2308-9" target="_blank" >https://link.springer.com/article/10.1007/s10404-019-2308-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10404-019-2308-9" target="_blank" >10.1007/s10404-019-2308-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Heat Transfer Time Determination Based on DNA Melting Curve Analysis

Popis výsledku v původním jazyce

The determination of the physical properties of fluids – such as the thermal characteristics, which include heat transfer time (Δt) – is becoming more challenging as system sizes shrink to micro and nanometer scales. Hence, knowledge of these properties is crucial for the operation of devices requiring precise temperature (T) control, such as polymerase chain reactions, melting curve analysis (MCA), and differential scanning fluorimetry. In this paper, we introduced a flow-through microfluidic system to analyze and compare thermal properties such as Δt among samples and the sidewall of a silicon chip using microscopic image analysis. We performed a spatial MCA with double-stranded deoxynucleic acid (dsDNA) and EvaGreen intercalator, using a flow-through microfluidic chip, and achieved a T gradient of ≈ 2.23 K·mm−1. We calculated the mean value of Δt as ≈ 33.9 ms from a melting temperature (TM) location shift along the microchannel for a variable flow rate. Our system had a T resolution of ≈ 1.2 mK·pixel-1 to distinguish different dsDNA molecules – based on the TM location within the chip – providing an option to use it as a high-throughput device for rapid DNA or protein analysis.

Název v anglickém jazyce

Heat Transfer Time Determination Based on DNA Melting Curve Analysis

Popis výsledku anglicky

The determination of the physical properties of fluids – such as the thermal characteristics, which include heat transfer time (Δt) – is becoming more challenging as system sizes shrink to micro and nanometer scales. Hence, knowledge of these properties is crucial for the operation of devices requiring precise temperature (T) control, such as polymerase chain reactions, melting curve analysis (MCA), and differential scanning fluorimetry. In this paper, we introduced a flow-through microfluidic system to analyze and compare thermal properties such as Δt among samples and the sidewall of a silicon chip using microscopic image analysis. We performed a spatial MCA with double-stranded deoxynucleic acid (dsDNA) and EvaGreen intercalator, using a flow-through microfluidic chip, and achieved a T gradient of ≈ 2.23 K·mm−1. We calculated the mean value of Δt as ≈ 33.9 ms from a melting temperature (TM) location shift along the microchannel for a variable flow rate. Our system had a T resolution of ≈ 1.2 mK·pixel-1 to distinguish different dsDNA molecules – based on the TM location within the chip – providing an option to use it as a high-throughput device for rapid DNA or protein analysis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microfluidics and Nanofluidics

ISSN

1613-4982

e-ISSN

1613-4990

Svazek periodika

neuveden

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

1-7

Kód UT WoS článku

000502437900001

EID výsledku v databázi Scopus

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