Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F11%3A%230000346" target="_blank" >RIV/14864347:_____/11:#0000346 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.brewingscience.de/abstracts.php3" target="_blank" >http://www.brewingscience.de/abstracts.php3</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1

Popis výsledku v původním jazyce

Complex lupulin gland-specific cDNA library from Osvald's clone 72 hop was constructed to dissect regulation of biosynthetic pathway(s) leading to the accumulation of hop bitter acids and prenylflavonoids with promising health-beneficial activities. Fromthis cDNA library we isolated first hop-specific allelic isoforms of transcription factors (TF) bHLH (HlbHLH2, GeneBank AC:FR751553) and WD40 (HlWD40_1, AC:NM_122360) which are involved in combinatorial control of light-responsive and tissue-specific activation of phenylpropanoid pathway. HlbHLH2 and HlWD40_1 are quite lupulin gland-specific and, according to our transient expression experiments, they form specific complexes with another novel hop TF HlMyb2 (AC:FN646081) and previously isolated lupulingland-specific HlMyb3 (AC:AM501509). The complex formation leads to strong activation of chalconsynthase gene (chs_H1) promoter. The interplay and regulation of expression of these crucial TF complexes could co-determine the rate of accu

Název v anglickém jazyce

Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1

Popis výsledku anglicky

Complex lupulin gland-specific cDNA library from Osvald's clone 72 hop was constructed to dissect regulation of biosynthetic pathway(s) leading to the accumulation of hop bitter acids and prenylflavonoids with promising health-beneficial activities. Fromthis cDNA library we isolated first hop-specific allelic isoforms of transcription factors (TF) bHLH (HlbHLH2, GeneBank AC:FR751553) and WD40 (HlWD40_1, AC:NM_122360) which are involved in combinatorial control of light-responsive and tissue-specific activation of phenylpropanoid pathway. HlbHLH2 and HlWD40_1 are quite lupulin gland-specific and, according to our transient expression experiments, they form specific complexes with another novel hop TF HlMyb2 (AC:FN646081) and previously isolated lupulingland-specific HlMyb3 (AC:AM501509). The complex formation leads to strong activation of chalconsynthase gene (chs_H1) promoter. The interplay and regulation of expression of these crucial TF complexes could co-determine the rate of accu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Brewing Science

ISSN

0723-1520

e-ISSN

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Svazek periodika

64

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

151-155

Kód UT WoS článku

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EID výsledku v databázi Scopus

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