Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00375145" target="_blank" >RIV/60077344:_____/11:00375145 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1

Popis výsledku v původním jazyce

Complex lupulin gland-specific cDNA library from Osvald?s clone 72 hop was constructed to dissect regulation of biosynthetic pathway(s) leading to the accumulation of hop bitter acids and prenylflavonoids with promising health-beneficial activities. Fromthis cDNA library we isolated first hop-specific allelic isoforms of transcription factors (TF) HlbHLH2 and HlWDR1 which are involved in combinatorial control of lightresponsive and tissue-specific activation of phenylpropanoid pathway. HlbHLH2 and HlWDR1 are quite lupulin gland-specific and, according to our transient expression experiments, they form specific complexes with another novel hop TF HlMyb2 and previously isolated lupulin glandspecific HlMyb3. The complex formation leads to strong activation of chalconsynthase gene (chs_H1) promoter. The interplay and regulation of expression of these crucial TF complexes could co-determine the rate of accumulation of valuable metabolites of lupulin.

Název v anglickém jazyce

Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1

Popis výsledku anglicky

Complex lupulin gland-specific cDNA library from Osvald?s clone 72 hop was constructed to dissect regulation of biosynthetic pathway(s) leading to the accumulation of hop bitter acids and prenylflavonoids with promising health-beneficial activities. Fromthis cDNA library we isolated first hop-specific allelic isoforms of transcription factors (TF) HlbHLH2 and HlWDR1 which are involved in combinatorial control of lightresponsive and tissue-specific activation of phenylpropanoid pathway. HlbHLH2 and HlWDR1 are quite lupulin gland-specific and, according to our transient expression experiments, they form specific complexes with another novel hop TF HlMyb2 and previously isolated lupulin glandspecific HlMyb3. The complex formation leads to strong activation of chalconsynthase gene (chs_H1) promoter. The interplay and regulation of expression of these crucial TF complexes could co-determine the rate of accumulation of valuable metabolites of lupulin.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Brewing Science

ISSN

1613-2041

e-ISSN

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Svazek periodika

64

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

151-155

Kód UT WoS článku

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EID výsledku v databázi Scopus

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