Comparative transcriptome analysis of Humulus lupulus wild-type and transformant co-expressing HIWRKY1 nad HIWDR1 transcriptional factors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F17%3AN0000070" target="_blank" >RIV/14864347:_____/17:N0000070 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.lfl.bayern.de/ipz/hopfen/024299/" target="_blank" >http://www.lfl.bayern.de/ipz/hopfen/024299/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparative transcriptome analysis of Humulus lupulus wild-type and transformant co-expressing HIWRKY1 nad HIWDR1 transcriptional factors

Popis výsledku v původním jazyce

MISHRA, Ajay Kumar, G. S. DURAISAMY, Mudra KHARE, Tomáš KOCÁBEK, Jernej JAKŠE, J. BŘÍZA, Josef PATZAK, T. SANO and Jaroslav MATOUŠEK. Comparative transcriptome analysis of Humulus lupulus wild-type and transformant co-expressing HIWRKY1 nad HIWDR1 transcriptional factors. In: F. WEIHRAUCH, ed. Proceedings of the Scientific-Technical Commission 25 – 29 June 2017, St. Stefan am Walde, Austria. Wolnzach: Scientific-Technical Commission of the International Hop Growers´ Convention, 2017, pp. 85–86. ISSN 2512-3785. Hop (Humulus lupulus L., Cannabaceae) is a dioecious, twining perennial flowering plant, native to Europe, western Asia and North America. The lupulin glands, which are glandular trichomes present on hop cones (high density) and leaves (low density) are composed of biosynthetic cells. They secret a specific but complex metabolome consisting mainly of terpenophenolics (hop bitter acids and prenylflavonoids) and terpenoids (essential oil components), which serve as essential raw ingredient in beer, contributing the distinctive bitterness, flavour and aroma, as well as preservative activity (MATOUŠEK et al. 2002; NAGEL et al. 2008). The terminal step of biosynthesis of prenylated flavonoids in hop cones is mediated by chalcone synthase CHS_H1, prenyltransferase (Prt) and O-methyltransferase 1 (omt1) enzymes (MATOUŠEK et al. 2013). Recently, we have shown that lupulin gland-specific transcription factor HlWRKY1, close homologue of AtWRKY75 in the binary combination of WD40 repeat protein1 (HlWDR1) activate the promoters of genes (HlOmt1, HlChs_H1, HlPrt1(2) and HlVps) involved in the final steps of prenylflavonoid (PF) and bitter acid (BA) biosynthesis pathway (MATOUŠEK et al. 2016).

Název v anglickém jazyce

Comparative transcriptome analysis of Humulus lupulus wild-type and transformant co-expressing HIWRKY1 nad HIWDR1 transcriptional factors

Popis výsledku anglicky

MISHRA, Ajay Kumar, G. S. DURAISAMY, Mudra KHARE, Tomáš KOCÁBEK, Jernej JAKŠE, J. BŘÍZA, Josef PATZAK, T. SANO and Jaroslav MATOUŠEK. Comparative transcriptome analysis of Humulus lupulus wild-type and transformant co-expressing HIWRKY1 nad HIWDR1 transcriptional factors. In: F. WEIHRAUCH, ed. Proceedings of the Scientific-Technical Commission 25 – 29 June 2017, St. Stefan am Walde, Austria. Wolnzach: Scientific-Technical Commission of the International Hop Growers´ Convention, 2017, pp. 85–86. ISSN 2512-3785. Hop (Humulus lupulus L., Cannabaceae) is a dioecious, twining perennial flowering plant, native to Europe, western Asia and North America. The lupulin glands, which are glandular trichomes present on hop cones (high density) and leaves (low density) are composed of biosynthetic cells. They secret a specific but complex metabolome consisting mainly of terpenophenolics (hop bitter acids and prenylflavonoids) and terpenoids (essential oil components), which serve as essential raw ingredient in beer, contributing the distinctive bitterness, flavour and aroma, as well as preservative activity (MATOUŠEK et al. 2002; NAGEL et al. 2008). The terminal step of biosynthesis of prenylated flavonoids in hop cones is mediated by chalcone synthase CHS_H1, prenyltransferase (Prt) and O-methyltransferase 1 (omt1) enzymes (MATOUŠEK et al. 2013). Recently, we have shown that lupulin gland-specific transcription factor HlWRKY1, close homologue of AtWRKY75 in the binary combination of WD40 repeat protein1 (HlWDR1) activate the promoters of genes (HlOmt1, HlChs_H1, HlPrt1(2) and HlVps) involved in the final steps of prenylflavonoid (PF) and bitter acid (BA) biosynthesis pathway (MATOUŠEK et al. 2016).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/GA13-03037S" target="_blank" >GA13-03037S: Kombinační regulace a regulační síť transkripčních faktorů účastnících se biosyntézy ozdravných prenylflavonoidů chmelu (Humulus lupulus L.).</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů