The introduction to gene network influencing lupulin and some approaches to molecular study of hop metabolome regulation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F17%3AN0000068" target="_blank" >RIV/14864347:_____/17:N0000068 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.lfl.bayern.de/ipz/hopfen/024299/" target="_blank" >http://www.lfl.bayern.de/ipz/hopfen/024299/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The introduction to gene network influencing lupulin and some approaches to molecular study of hop metabolome regulation

Popis výsledku v původním jazyce

MATOUŠEK, Jaroslav, Ajay Kumar MISHRA, Tomáš KOCÁBEK, Josef PATZAK, Mudra KHARE, G. S. DURAISAMY, Petr SVOBODA and Karel KROFTA. The introduction to gene network influencing lupulin and some approaches to molecular study of hop metabolome regulation. In: F. WEIHRAUCH, ed. Proceedings of the Scientific-Technical Commission 25 – 29 June 2017, St. Stefan am Walde, Austria. Wolnzach: Scientific-Technical Commission of the International Hop Growers´ Convention, 2017, pp. 82–83. ISSN 2512-3785. In our previous work we characterized several transcription factors (TFs) and TFs complexes that activate or suppress hop (Humulus lupulus L.) lupulin-specific genes involved in the regulation of prenylflavonoids. Among them there are regulatory genes from Myb, bHLH, WRKY, WD40 and bZIP families. The purpose of our work during several past years was to analyze some network connections especially within regulation of the final steps of prenylflavonoid biosynthesis involving genes encoding enzymes like chalcone synthase CHS_H1, prenyltransferase (Prt) and O-methyltransferase 1 (omt1). Several specific TF genes like HlMyb3 (e.g., MATOUŠEK et al. 2007; GATICA-ARIAS et al. 2013), HlMyb7 (MATOUŠEK et al. 2012), HlMyb8 (unpub.) and TF complexes like HlMyb3/HlbHLH2/HlWDR1, HlMyb2/HlbHLH2/HlWDR1 (MATOUŠEK et al. 2012) and HlWRKY1/HlWDR1 (WW) (MATOUŠEK et al. 2016) are of special interest and they were mapped in the initial regulátory network. In the case of WW complexes we described wide dependency of HlWRKY1 on phosphorylation, autoactivation and RNA silencing (MATOUŠEK et al. 2016). According to our recent analyses hop gene modulation by tripartite complexes is, in addition, strongly dependent on experimental light conditions enriched either by blue, red or UV-A spectra. Thus, all factors analysed so far suggest high complexity of lupulin regulatory network.

Název v anglickém jazyce

The introduction to gene network influencing lupulin and some approaches to molecular study of hop metabolome regulation

Popis výsledku anglicky

MATOUŠEK, Jaroslav, Ajay Kumar MISHRA, Tomáš KOCÁBEK, Josef PATZAK, Mudra KHARE, G. S. DURAISAMY, Petr SVOBODA and Karel KROFTA. The introduction to gene network influencing lupulin and some approaches to molecular study of hop metabolome regulation. In: F. WEIHRAUCH, ed. Proceedings of the Scientific-Technical Commission 25 – 29 June 2017, St. Stefan am Walde, Austria. Wolnzach: Scientific-Technical Commission of the International Hop Growers´ Convention, 2017, pp. 82–83. ISSN 2512-3785. In our previous work we characterized several transcription factors (TFs) and TFs complexes that activate or suppress hop (Humulus lupulus L.) lupulin-specific genes involved in the regulation of prenylflavonoids. Among them there are regulatory genes from Myb, bHLH, WRKY, WD40 and bZIP families. The purpose of our work during several past years was to analyze some network connections especially within regulation of the final steps of prenylflavonoid biosynthesis involving genes encoding enzymes like chalcone synthase CHS_H1, prenyltransferase (Prt) and O-methyltransferase 1 (omt1). Several specific TF genes like HlMyb3 (e.g., MATOUŠEK et al. 2007; GATICA-ARIAS et al. 2013), HlMyb7 (MATOUŠEK et al. 2012), HlMyb8 (unpub.) and TF complexes like HlMyb3/HlbHLH2/HlWDR1, HlMyb2/HlbHLH2/HlWDR1 (MATOUŠEK et al. 2012) and HlWRKY1/HlWDR1 (WW) (MATOUŠEK et al. 2016) are of special interest and they were mapped in the initial regulátory network. In the case of WW complexes we described wide dependency of HlWRKY1 on phosphorylation, autoactivation and RNA silencing (MATOUŠEK et al. 2016). According to our recent analyses hop gene modulation by tripartite complexes is, in addition, strongly dependent on experimental light conditions enriched either by blue, red or UV-A spectra. Thus, all factors analysed so far suggest high complexity of lupulin regulatory network.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/GA13-03037S" target="_blank" >GA13-03037S: Kombinační regulace a regulační síť transkripčních faktorů účastnících se biosyntézy ozdravných prenylflavonoidů chmelu (Humulus lupulus L.).</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů