Proliferation and cold hardening of in vitro grown apple shoot tips

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F06%3A0R000945" target="_blank" >RIV/25271121:_____/06:0R000945 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Proliferation and cold hardening of in vitro grown apple shoot tips

Popis výsledku v původním jazyce

Shoot tips of apple cv. Rubin were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg /L BAP and 0.1 mg /L IBA were tested. Thehighest proliferation rate was obtained on MS medium with 1 mg/ L BAP, which produced 4.2 +/- 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. Rubin were cultivated for 4 weeks at low temperature (4 degrees C) and short photoperiod (8/16-h - light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. The lethal temperature (LT) was 13.0 degrees C for cold hardened and -6.5 degrees C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of cv. Rubin was 78% after thawing in 40 degre

Název v anglickém jazyce

Proliferation and cold hardening of in vitro grown apple shoot tips

Popis výsledku anglicky

Shoot tips of apple cv. Rubin were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg /L BAP and 0.1 mg /L IBA were tested. Thehighest proliferation rate was obtained on MS medium with 1 mg/ L BAP, which produced 4.2 +/- 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. Rubin were cultivated for 4 weeks at low temperature (4 degrees C) and short photoperiod (8/16-h - light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. The lethal temperature (LT) was 13.0 degrees C for cold hardened and -6.5 degrees C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of cv. Rubin was 78% after thawing in 40 degre

Druh

D - Stať ve sborníku

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/QC1052" target="_blank" >QC1052: Nová metoda konzervace genetických zdrojů ovocných rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

978-90-6605-719-7

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

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Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven

Místo konání akce

Debrecen

Datum konání akce

12. 9. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000245292600066