In Vitro Multiplication of Old Pear Cultivars

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F15%3A%230000610" target="_blank" >RIV/25271121:_____/15:#0000610 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In Vitro Multiplication of Old Pear Cultivars

Popis výsledku v původním jazyce

In 2012, a project was started to evaluate the resistance of pear landraces and older cultivars grown in the Czech Republic to fire blight. A key part of the project was also testing of applicability of different in vitro culture media and methods of artificial inoculation under in vitro culture conditions. Therefore, the purpose of this study was to develop an efficient protocol for rapid in vitro shoot multiplication of older pear cultivars and landraces. The donor shoots were obtained in March from mature trees grown at the Research and Breeding Institute of Pomology (RBIP) Holovousy Ltd., Department of Genebanks (Holovousy 1, 50801, Horice, Czech Republic). Selected genotypes were successfully established in vitro using mercuric chloride as sterilization solution. For initiation of multiplication, five Murashige and Skoog (MS) media containing 1, 2 and 4 mg L-1 BAP (6-benzylaminopurine) or 0.5 and 1 mg L-1 TDZ (thidiazuron) were evaluated. Multiplication rate was defined as the number of newly formed shoots (>10 mm) per initial shoot tip after four weeks of culture. The effect of the two growth regulators on callus formation and shoot morphologywas determined. Selected pear cultivars differed in their multiplication and development potential on the evaluated media. Generally, the highest proliferation rate was obtained for pear cultivar General JLeclerc that produced 7.3 in vitro shoots (>10 mm) on MS medium containing 1 mg L-1 TDZ. Results obtained in our study confirmed preliminary findings that TDZ was an important plant growth regulator for proliferation and growth in pear micropropagation.

Název v anglickém jazyce

In Vitro Multiplication of Old Pear Cultivars

Popis výsledku anglicky

In 2012, a project was started to evaluate the resistance of pear landraces and older cultivars grown in the Czech Republic to fire blight. A key part of the project was also testing of applicability of different in vitro culture media and methods of artificial inoculation under in vitro culture conditions. Therefore, the purpose of this study was to develop an efficient protocol for rapid in vitro shoot multiplication of older pear cultivars and landraces. The donor shoots were obtained in March from mature trees grown at the Research and Breeding Institute of Pomology (RBIP) Holovousy Ltd., Department of Genebanks (Holovousy 1, 50801, Horice, Czech Republic). Selected genotypes were successfully established in vitro using mercuric chloride as sterilization solution. For initiation of multiplication, five Murashige and Skoog (MS) media containing 1, 2 and 4 mg L-1 BAP (6-benzylaminopurine) or 0.5 and 1 mg L-1 TDZ (thidiazuron) were evaluated. Multiplication rate was defined as the number of newly formed shoots (>10 mm) per initial shoot tip after four weeks of culture. The effect of the two growth regulators on callus formation and shoot morphologywas determined. Selected pear cultivars differed in their multiplication and development potential on the evaluated media. Generally, the highest proliferation rate was obtained for pear cultivar General JLeclerc that produced 7.3 in vitro shoots (>10 mm) on MS medium containing 1 mg L-1 TDZ. Results obtained in our study confirmed preliminary findings that TDZ was an important plant growth regulator for proliferation and growth in pear micropropagation.

Druh

D - Stať ve sborníku

CEP obor

GC - Pěstování rostlin, osevní postupy

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1210184" target="_blank" >QJ1210184: Využití biotechnologických metod pro zefektivnění testování rezistence jádrovin vůči patogenu bakteriální spály růžovitých (bakterie Erwinia amylovora)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

978-94-6261-092-7

ISSN

0567-7572

e-ISSN

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Počet stran výsledku

5

Strana od-do

163-167

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven, Belgium

Místo konání akce

Leuven, Belgium

Datum konání akce

14. 7. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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