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Protocol for in vitro shoot multiplication of ancient pear cultivars and landraces

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F21%3AN0000008" target="_blank" >RIV/25271121:_____/21:N0000008 - isvavai.cz</a>

  • Výsledek na webu

    <a href="http://dx.doi.org/10.17660/ActaHortic.2021.1303.15 " target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2021.1303.15 </a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.17660/ActaHortic.2021.1303.15 " target="_blank" >10.17660/ActaHortic.2021.1303.15 </a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Protocol for in vitro shoot multiplication of ancient pear cultivars and landraces

  • Popis výsledku v původním jazyce

    In 2016, a project was started to preserve the diversity of older pear cultivars grown in the Czech Republic. The purpose of this study was to develop an efficient in vitro system for rapid propagation of pear explants as initial plant material for cryopreservation experiments. The donor shoots were obtained in March from mature trees grown at the Research and Breeding Institute of Pomology (RBIP) Holovousy Ltd., Department of Genebanks. Selected pear genotypes ‘Avranches’, ‘Jakobibirne’, ‘Marillat’, ‘Flamandka’ were successfully established in vitro using 0.15 % mercuric chloride as disinfectant. Six MS-based media containing 1, 2 or 4 mg L-1 zeatin or 1, 2 or 4 mg L-1 BAP (6-benzylaminopurine) were used for multiplication experiments. Multiplication rate was defined as the number of newly formed shoots (10 mm) per initial shoot tip after four weeks of culture. Cultivars in the study differed in their proliferation and development potential in MS medium according to hormone level between 1.0 and 3.8. Generally, the highest proliferation rate (3.8) in our experiments was obtained for cultivar ‘Marillat’ on MS medium with 2 mg L-1 BAP. Sufficient multiplication at a rate of 3.1 was obtained only for ‘Marillat’ on a medium with the highest concentration cytokinin zeatin of 4 mg L-1. Results obtained in our study confirmed preliminary findings that BAP was an important plant growth regulator for proliferation and growth in pear micropropagation. During the in vitro multiplication phase, for all tested cultivars, no morphological abnormalities such as excessive callus formation, hyperhydricity or production of abnormally narrow leaves were noted.

  • Název v anglickém jazyce

    Protocol for in vitro shoot multiplication of ancient pear cultivars and landraces

  • Popis výsledku anglicky

    In 2016, a project was started to preserve the diversity of older pear cultivars grown in the Czech Republic. The purpose of this study was to develop an efficient in vitro system for rapid propagation of pear explants as initial plant material for cryopreservation experiments. The donor shoots were obtained in March from mature trees grown at the Research and Breeding Institute of Pomology (RBIP) Holovousy Ltd., Department of Genebanks. Selected pear genotypes ‘Avranches’, ‘Jakobibirne’, ‘Marillat’, ‘Flamandka’ were successfully established in vitro using 0.15 % mercuric chloride as disinfectant. Six MS-based media containing 1, 2 or 4 mg L-1 zeatin or 1, 2 or 4 mg L-1 BAP (6-benzylaminopurine) were used for multiplication experiments. Multiplication rate was defined as the number of newly formed shoots (10 mm) per initial shoot tip after four weeks of culture. Cultivars in the study differed in their proliferation and development potential in MS medium according to hormone level between 1.0 and 3.8. Generally, the highest proliferation rate (3.8) in our experiments was obtained for cultivar ‘Marillat’ on MS medium with 2 mg L-1 BAP. Sufficient multiplication at a rate of 3.1 was obtained only for ‘Marillat’ on a medium with the highest concentration cytokinin zeatin of 4 mg L-1. Results obtained in our study confirmed preliminary findings that BAP was an important plant growth regulator for proliferation and growth in pear micropropagation. During the in vitro multiplication phase, for all tested cultivars, no morphological abnormalities such as excessive callus formation, hyperhydricity or production of abnormally narrow leaves were noted.

Klasifikace

  • Druh

    D - Stať ve sborníku

  • CEP obor

  • OECD FORD obor

    40401 - Agricultural biotechnology and food biotechnology

Návaznosti výsledku

  • Projekt

    <a href="/cs/project/LO1608" target="_blank" >LO1608: Výzkumné ovocnářské centrum</a><br>

  • Návaznosti

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2021

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název statě ve sborníku

    Acta Horticulturae

  • ISBN

  • ISSN

    0567-7572

  • e-ISSN

  • Počet stran výsledku

    6

  • Strana od-do

    101-106

  • Název nakladatele

    ISHS

  • Místo vydání

    Leuven

  • Místo konání akce

    Montevideo; Uruguay

  • Datum konání akce

    3. 12. 2018

  • Typ akce podle státní příslušnosti

    WRD - Celosvětová akce

  • Kód UT WoS článku

    000706559200015