First Report of Prunus virus F Infecting Sour Cherry in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F17%3AN0000119" target="_blank" >RIV/25271121:_____/17:N0000119 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1094/PDIS-04-17-0469-PDN" target="_blank" >http://dx.doi.org/10.1094/PDIS-04-17-0469-PDN</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1094/PDIS-04-17-0469-PDN" target="_blank" >10.1094/PDIS-04-17-0469-PDN</a>

Jazyk výsledku

angličtina

Název v původním jazyce

First Report of Prunus virus F Infecting Sour Cherry in the Czech Republic

Popis výsledku v původním jazyce

Prunus virus F (PrVF) is new tentative species in the genus Fabavirus, first described infecting stone fruits of temperate regions. It has recently been described in the complex of viruses causing sweet cherry tree decline in the U.S.A. During a survey for Prunus spp. viruses in the Czech Republic carried out in June 2008, three asymptomatic samples of sour cherry (P. cerasus) cv. Amarelka chvalkovicka were collected (among others) in a germplasm collection in Holovousy. CLC Genomics Workbench assembly of Illumina deep sequencing reads obtained from double-stranded RNAs isolated from leaves of these trees generated 21, 25, and 12 PrVF specific contigs totaling 7.38, 5.69, and 3.48 kb for each sample, respectively. The presence of PrVF in all three trees was confirmed by RT-PCR, in both the original and newly collected samples, using primers PrVF1F (5′-TTGTTATGGGCCTCGCTCTG-3′) and PrVF1R (5′-ATCAGTTTGAAGGGAAGGCCA-3′), PrVF2F (5′-CAAGCTTTYCTTGGYGCTTT-3′) and PrVF2R (5′-GAACACGCTGGGTCATYTCA-3′) for RNA1 and RNA2 detection. Amplicons were cloned into the pGEM-T Easy plasmid and three colonies were sequenced for each of them. To our knowledge, this is the first report of PrVF infecting sour cherry in the Czech Republic. Further efforts are needed to evaluate the prevalence of PrVF in cherry populations, its mode(s) of transmission, and its potential pathogenicity.

Název v anglickém jazyce

First Report of Prunus virus F Infecting Sour Cherry in the Czech Republic

Popis výsledku anglicky

Prunus virus F (PrVF) is new tentative species in the genus Fabavirus, first described infecting stone fruits of temperate regions. It has recently been described in the complex of viruses causing sweet cherry tree decline in the U.S.A. During a survey for Prunus spp. viruses in the Czech Republic carried out in June 2008, three asymptomatic samples of sour cherry (P. cerasus) cv. Amarelka chvalkovicka were collected (among others) in a germplasm collection in Holovousy. CLC Genomics Workbench assembly of Illumina deep sequencing reads obtained from double-stranded RNAs isolated from leaves of these trees generated 21, 25, and 12 PrVF specific contigs totaling 7.38, 5.69, and 3.48 kb for each sample, respectively. The presence of PrVF in all three trees was confirmed by RT-PCR, in both the original and newly collected samples, using primers PrVF1F (5′-TTGTTATGGGCCTCGCTCTG-3′) and PrVF1R (5′-ATCAGTTTGAAGGGAAGGCCA-3′), PrVF2F (5′-CAAGCTTTYCTTGGYGCTTT-3′) and PrVF2R (5′-GAACACGCTGGGTCATYTCA-3′) for RNA1 and RNA2 detection. Amplicons were cloned into the pGEM-T Easy plasmid and three colonies were sequenced for each of them. To our knowledge, this is the first report of PrVF infecting sour cherry in the Czech Republic. Further efforts are needed to evaluate the prevalence of PrVF in cherry populations, its mode(s) of transmission, and its potential pathogenicity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

<a href="/cs/project/LO1608" target="_blank" >LO1608: Výzkumné ovocnářské centrum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Disease

ISSN

0191-2917

e-ISSN

—

Svazek periodika

101

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

1

Strana od-do

1828

Kód UT WoS článku

000410877900039

EID výsledku v databázi Scopus

—