Variability Studies of Two Prunus-Infecting Fabaviruses with the Aid of High-Throughput Sequencing
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F18%3A00495082" target="_blank" >RIV/60077344:_____/18:00495082 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.3390/v10040204" target="_blank" >http://dx.doi.org/10.3390/v10040204</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/v10040204" target="_blank" >10.3390/v10040204</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Variability Studies of Two Prunus-Infecting Fabaviruses with the Aid of High-Throughput Sequencing
Popis výsledku v původním jazyce
During their lifetime, perennial woody plants are expected to face multiple infection events. Furthermore, multiple genotypes of individual virus species may co-infect the same host. This may eventually lead to a situation where plants harbor complex communities of viral species/strains. Using high-throughput sequencing, we describe co-infection of sweet and sour cherry trees with diverse genomic variants of two closely related viruses, namely prunus virus F (PrVF) and cherry virus F (CVF). Both viruses are most homologous to members of the Fabavirus genus (Secoviridae family). The comparison of CVF and PrVF RNA2 genomic sequences suggests that the two viruses may significantly differ in their expression strategy. Indeed, similar to comoviruses, the smaller genomic segment of PrVF, RNA2, may be translated in two collinear proteins while CVF likely expresses only the shorter of these two proteins. Linked with the observation that identity levels between the coat proteins of these two viruses are significantly below the family species demarcation cut-off, these findings support the idea that CVF and PrVF represent two separate Fabavirus species.
Název v anglickém jazyce
Variability Studies of Two Prunus-Infecting Fabaviruses with the Aid of High-Throughput Sequencing
Popis výsledku anglicky
During their lifetime, perennial woody plants are expected to face multiple infection events. Furthermore, multiple genotypes of individual virus species may co-infect the same host. This may eventually lead to a situation where plants harbor complex communities of viral species/strains. Using high-throughput sequencing, we describe co-infection of sweet and sour cherry trees with diverse genomic variants of two closely related viruses, namely prunus virus F (PrVF) and cherry virus F (CVF). Both viruses are most homologous to members of the Fabavirus genus (Secoviridae family). The comparison of CVF and PrVF RNA2 genomic sequences suggests that the two viruses may significantly differ in their expression strategy. Indeed, similar to comoviruses, the smaller genomic segment of PrVF, RNA2, may be translated in two collinear proteins while CVF likely expresses only the shorter of these two proteins. Linked with the observation that identity levels between the coat proteins of these two viruses are significantly below the family species demarcation cut-off, these findings support the idea that CVF and PrVF represent two separate Fabavirus species.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10607 - Virology
Návaznosti výsledku
Projekt
<a href="/cs/project/LD15035" target="_blank" >LD15035: Jak využívat a interpretovat přebytek dat z paralelního sekvenování v rostlinné virologii?</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Viruses
ISSN
1999-4915
e-ISSN
—
Svazek periodika
10
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
13
Strana od-do
—
Kód UT WoS článku
000435184400065
EID výsledku v databázi Scopus
2-s2.0-85046092795