Gene expression of Mal d 1 allergen isoforms in apple fruits determined using new generation sequencing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F21%3AN0000017" target="_blank" >RIV/25271121:_____/21:N0000017 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2021.1307.35" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2021.1307.35</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2021.1307.35" target="_blank" >10.17660/ActaHortic.2021.1307.35</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Gene expression of Mal d 1 allergen isoforms in apple fruits determined using new generation sequencing

Popis výsledku v původním jazyce

Apples are one of the most consumed fruit species. However, eating fresh apples can cause an allergic reaction in a certain percentage of population. We evaluated the gene expression of Mal d 1, a major apple allergen in Central and Northern Europe, in four cultivars: ‘Idared’, UEB 32642, ‘Topaz’, and ‘Rubín’. Fruits of ‘Idared’, ‘Topaz’ and ‘Rubín’, were freshly harvested, while fruits of UEB 32642 were stored for four months in two ways: (i) under ultra-low oxygen conditions and (ii) under cold storage conditions. Total RNA was isolated from the apples and transcribed into cDNA. We applied the new generation sequencing (NGS) method using the Ion Torrent Personal Genome Machine (Ion PGM) sequencer to quantify the gene expression of all 31 allergen isoforms. Out of the tested allergen isoforms, relative gene expression exceeding 1% was observed only for seven genes, namely Mal d 1.01, Mal d 1.02, Mal d 1.05, Mal d 1.06A, Mal d 1.06B, Mal d 1.13A, and Mal d 1.13C. The expression of these genes accounted for 95% of all allergens contained at varying rates in the apples. However, only three genes, Mal d 1.01, Mal d 1.02 and Mal d 1.06A, dominated in all cultivars and represented 88-98% of all allergens of the cultivar. In contrast, almost no expression at background level was observed for three genes Mal d 1.04, Mal d 1.12 and Mal d 1.14.

Název v anglickém jazyce

Gene expression of Mal d 1 allergen isoforms in apple fruits determined using new generation sequencing

Popis výsledku anglicky

Apples are one of the most consumed fruit species. However, eating fresh apples can cause an allergic reaction in a certain percentage of population. We evaluated the gene expression of Mal d 1, a major apple allergen in Central and Northern Europe, in four cultivars: ‘Idared’, UEB 32642, ‘Topaz’, and ‘Rubín’. Fruits of ‘Idared’, ‘Topaz’ and ‘Rubín’, were freshly harvested, while fruits of UEB 32642 were stored for four months in two ways: (i) under ultra-low oxygen conditions and (ii) under cold storage conditions. Total RNA was isolated from the apples and transcribed into cDNA. We applied the new generation sequencing (NGS) method using the Ion Torrent Personal Genome Machine (Ion PGM) sequencer to quantify the gene expression of all 31 allergen isoforms. Out of the tested allergen isoforms, relative gene expression exceeding 1% was observed only for seven genes, namely Mal d 1.01, Mal d 1.02, Mal d 1.05, Mal d 1.06A, Mal d 1.06B, Mal d 1.13A, and Mal d 1.13C. The expression of these genes accounted for 95% of all allergens contained at varying rates in the apples. However, only three genes, Mal d 1.01, Mal d 1.02 and Mal d 1.06A, dominated in all cultivars and represented 88-98% of all allergens of the cultivar. In contrast, almost no expression at background level was observed for three genes Mal d 1.04, Mal d 1.12 and Mal d 1.14.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

<a href="/cs/project/LO1608" target="_blank" >LO1608: Výzkumné ovocnářské centrum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae, XV EUCARPIA Symposium on Fruit Breeding and Genetics

ISBN

—

ISSN

0567-7572

e-ISSN

—

Počet stran výsledku

4

Strana od-do

227-230

Název nakladatele

ISHS

Místo vydání

Leuven

Místo konání akce

Praha

Datum konání akce

3. 6. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000948137200034