Expression of Mal d 1 allergen genes in commercial apple cultivars

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F21%3AN0000020" target="_blank" >RIV/25271121:_____/21:N0000020 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2021.1307.61" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2021.1307.61</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2021.1307.61" target="_blank" >10.17660/ActaHortic.2021.1307.61</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression of Mal d 1 allergen genes in commercial apple cultivars

Popis výsledku v původním jazyce

Apple allergy is one of the most common types of food allergy. The main allergen Mal d 1 is a complex gene family of 31 loci, where each of the loci encodes a different isoallergen. Particular isoforms have varying levels of expression depending on many factors, both genetic and environmental. Here, the isoform expression of Mal d 1.01, Mal d 1.02, Mal d 1.06A, and Mal d 1.06B was studied. Differences in the expression of individual isoforms were evaluated, based on cultivar, pomology, origin, packaging, and quality grades. Furthermore, the differences between the expression of allergen genes in the fruit skin and the fruit flesh were evaluated. Relative gene expression was used as an evaluation method. The results show predominant expression of isoforms Mal d 1.01 and Mal d 1.02. Cultivars with highly expressed allergen genes include 'Golden Delicious' and 'Jonagold', on the other hand, low levels of expression were found in 'Modi'. Our findings also suggest that the fruit skin has higher allergenicity than the fruit flesh and the factors such as storage duration, storage method, fruit handling, packing, quality grades, and pathogenesis associated with defensive reactions of the fruit under unfavorable conditions cause higher expression of allergen genes. Our research presents a unique work because it involved a comprehensive approach assessing many factors related to fruit production, transportation, storage, and sales which could affect allergen gene expression.

Název v anglickém jazyce

Expression of Mal d 1 allergen genes in commercial apple cultivars

Popis výsledku anglicky

Apple allergy is one of the most common types of food allergy. The main allergen Mal d 1 is a complex gene family of 31 loci, where each of the loci encodes a different isoallergen. Particular isoforms have varying levels of expression depending on many factors, both genetic and environmental. Here, the isoform expression of Mal d 1.01, Mal d 1.02, Mal d 1.06A, and Mal d 1.06B was studied. Differences in the expression of individual isoforms were evaluated, based on cultivar, pomology, origin, packaging, and quality grades. Furthermore, the differences between the expression of allergen genes in the fruit skin and the fruit flesh were evaluated. Relative gene expression was used as an evaluation method. The results show predominant expression of isoforms Mal d 1.01 and Mal d 1.02. Cultivars with highly expressed allergen genes include 'Golden Delicious' and 'Jonagold', on the other hand, low levels of expression were found in 'Modi'. Our findings also suggest that the fruit skin has higher allergenicity than the fruit flesh and the factors such as storage duration, storage method, fruit handling, packing, quality grades, and pathogenesis associated with defensive reactions of the fruit under unfavorable conditions cause higher expression of allergen genes. Our research presents a unique work because it involved a comprehensive approach assessing many factors related to fruit production, transportation, storage, and sales which could affect allergen gene expression.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

40400 - Agricultural biotechnology

Projekt

<a href="/cs/project/LO1608" target="_blank" >LO1608: Výzkumné ovocnářské centrum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae, XV EUCARPIA Symposium on Fruit Breeding and Genetics

ISBN

—

ISSN

0567-7572

e-ISSN

—

Počet stran výsledku

6

Strana od-do

405 - 410

Název nakladatele

ISHS

Místo vydání

Leuven

Místo konání akce

Praha

Datum konání akce

3. 6. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000948137200060