Effect of long-term storage on the change in the expression of selected Mal d 1 gene isoforms in the apple cultivar Opal®

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F23%3AN0000128" target="_blank" >RIV/25271121:_____/23:N0000128 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/23:95160

Výsledek na webu

<a href="https://cjgpb.agriculturejournals.cz/artkey/cjg-202303-0003_effect-of-long-term-storage-on-the-change-in-the-expression-of-selected-mal-d-1-gene-isoforms-in-the-apple-cult.php" target="_blank" >https://cjgpb.agriculturejournals.cz/artkey/cjg-202303-0003_effect-of-long-term-storage-on-the-change-in-the-expression-of-selected-mal-d-1-gene-isoforms-in-the-apple-cult.php</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/102/2022-CJGPB" target="_blank" >10.17221/102/2022-CJGPB</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of long-term storage on the change in the expression of selected Mal d 1 gene isoforms in the apple cultivar Opal®

Popis výsledku v původním jazyce

Apples are available all year round since they can be stored for long time. However, the expression of the major apple allergen Mal d 1 can increase in the fruit during storage and incease so the risk of allergies in sensitive consumers. Therefore, we studied the change in the expression of the Mal d 1 gene during storage in the modern cultivar UEB 32642 (known under the brand name Opal®) and the cultivar Gala Brookfield (cv. Gala) as a reference. The cultivars were stored under two different conditions, ultra-low oxygen (ULO) and standard cold conditions. The gene expression was evaluated both in fresh fruits and in fruits stored for four, six, and nine months. Nine isoforms of the gene Mal d 1 with the highest published expression were quantified using real-time PCR. The most expressed isoforms Mal d 1.01, Mal d 1.02 and Mal d 1.06A were found in the fresh and also in the stored fruits. The expression of the Mal d 1.03G and Mal d 1.06D isoforms was higher the sored fruits. Our study confirmed that (i) Opal® had a lower overall expression of the Mal d 1 gene than cv. Gala, both in the fresh and stored fruits; (ii) standard cold storage is superior in preserving lower Mal d 1 levels in Opal®apples compared to the ULO conditions; and (iii) less expressed isoforms may be responsible for the general increase in the Mal d 1 gene expression during storage.

Název v anglickém jazyce

Effect of long-term storage on the change in the expression of selected Mal d 1 gene isoforms in the apple cultivar Opal®

Popis výsledku anglicky

Apples are available all year round since they can be stored for long time. However, the expression of the major apple allergen Mal d 1 can increase in the fruit during storage and incease so the risk of allergies in sensitive consumers. Therefore, we studied the change in the expression of the Mal d 1 gene during storage in the modern cultivar UEB 32642 (known under the brand name Opal®) and the cultivar Gala Brookfield (cv. Gala) as a reference. The cultivars were stored under two different conditions, ultra-low oxygen (ULO) and standard cold conditions. The gene expression was evaluated both in fresh fruits and in fruits stored for four, six, and nine months. Nine isoforms of the gene Mal d 1 with the highest published expression were quantified using real-time PCR. The most expressed isoforms Mal d 1.01, Mal d 1.02 and Mal d 1.06A were found in the fresh and also in the stored fruits. The expression of the Mal d 1.03G and Mal d 1.06D isoforms was higher the sored fruits. Our study confirmed that (i) Opal® had a lower overall expression of the Mal d 1 gene than cv. Gala, both in the fresh and stored fruits; (ii) standard cold storage is superior in preserving lower Mal d 1 levels in Opal®apples compared to the ULO conditions; and (iii) less expressed isoforms may be responsible for the general increase in the Mal d 1 gene expression during storage.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

CZECH JOURNAL OF GENETICS AND PLANT BREEDING

ISSN

1212-1975

e-ISSN

—

Svazek periodika

59

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

141-147

Kód UT WoS článku

001088810400002

EID výsledku v databázi Scopus

2-s2.0-85163326738