In Vitro Evaluation of Prebiotics on Adherence of Lactobacilli

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F16%3AN0000072" target="_blank" >RIV/26722861:_____/16:N0000072 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.omicsonline.org/open-access/in-vitro-evaluation-of-prebiotics-on-adherence-of-lactobacilli-1948-5948-1000253.pdf" target="_blank" >https://www.omicsonline.org/open-access/in-vitro-evaluation-of-prebiotics-on-adherence-of-lactobacilli-1948-5948-1000253.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4172/1948-5948.1000253" target="_blank" >10.4172/1948-5948.1000253</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In Vitro Evaluation of Prebiotics on Adherence of Lactobacilli

Popis výsledku v původním jazyce

The objective of this study was to test the effect of three commercially available prebiotics: Orafti GR, Orafti P95, and Orafti Synergy on the adherence of five Lactobacillus strains (Lactobacillus delbrueckii subsp. bulgaricus CCDM 66, Lactobacillus casei subsp. paracasei PE1TB-P, Lactobacillus fermentum RL25, Lactobacillus animalis CCDM 382 and Lactobacillus gasseri PHM-7E1). Adherence was tested using microtiter plates and was evaluated as the percentage of fluorescently labelled bacteria present in the wells after three washes. Adherence was evaluated in both uncoated and mucin-coated polystyrene plates. In accordance with our previous results, the majority of probiotic and prebiotic interactions resulted in at least a 10-fold decrease in adherence. Only one strain, L. gasseri PHM-7E1, exhibited increased adherence to mucin (from 5 % to 8 % and from 5 % to 9 %) after the addition of the prebiotics Orafti P95 and GR, respectively. These two combinations appear suitable for further synbiotic testing.

Název v anglickém jazyce

In Vitro Evaluation of Prebiotics on Adherence of Lactobacilli

Popis výsledku anglicky

The objective of this study was to test the effect of three commercially available prebiotics: Orafti GR, Orafti P95, and Orafti Synergy on the adherence of five Lactobacillus strains (Lactobacillus delbrueckii subsp. bulgaricus CCDM 66, Lactobacillus casei subsp. paracasei PE1TB-P, Lactobacillus fermentum RL25, Lactobacillus animalis CCDM 382 and Lactobacillus gasseri PHM-7E1). Adherence was tested using microtiter plates and was evaluated as the percentage of fluorescently labelled bacteria present in the wells after three washes. Adherence was evaluated in both uncoated and mucin-coated polystyrene plates. In accordance with our previous results, the majority of probiotic and prebiotic interactions resulted in at least a 10-fold decrease in adherence. Only one strain, L. gasseri PHM-7E1, exhibited increased adherence to mucin (from 5 % to 8 % and from 5 % to 9 %) after the addition of the prebiotics Orafti P95 and GR, respectively. These two combinations appear suitable for further synbiotic testing.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LD14123" target="_blank" >LD14123: Sledování adheze a tvorby biofilmů u probiotických bakterií a vliv prebiotik na jejich adhezi.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbial and Biochemical Technology

ISSN

1948-5948

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

3

Strana od-do

450-452

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—