Adherence to Caco-2 and HT-29-MTX cell lines, autoaggregation and hydrophobicity of novel, potentially probiotic strains of lactobacilli and bifidobacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F18%3AN0000013" target="_blank" >RIV/26722861:_____/18:N0000013 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Adherence to Caco-2 and HT-29-MTX cell lines, autoaggregation and hydrophobicity of novel, potentially probiotic strains of lactobacilli and bifidobacteria

Popis výsledku v původním jazyce

More than 200 isolates were obtained from samples of cow colostrum, excrements and saliva of calves and piglets as well as infant faeces. The isolates were identified and a narrower set of lactobacilli and bifidobacteria has been chosen for further testing, where some of their properties having an affinity to adhesion, adherence to human cells, surface hydrophobicity or auto-aggregation were investigated. Positive interplay of all properties important to adhesion provides the benefit of probiotic strains in colonization of the intestine. The most freqeuntly isolated strain was Lactobacillus reuteri and Lactobacillus casei subsp. paracasei. Bifidobacteria were isolated only from calves and piglets (B. thermophilum and B. pseudolongum) and B. longum from calf saliva. Generally, all tested isolates were able to adhere to the tissue model in in vitro conditions. The highest adherence capacity was found in the strain K7 Lacobacillus amylovorus (37,22%). Adherence above 30% was determined primarily in strains isolated from infant faeces (E3TD, E2MD, E3TA and E3M2), also from cow colostrum (K14 and K7).

Název v anglickém jazyce

Adherence to Caco-2 and HT-29-MTX cell lines, autoaggregation and hydrophobicity of novel, potentially probiotic strains of lactobacilli and bifidobacteria

Popis výsledku anglicky

More than 200 isolates were obtained from samples of cow colostrum, excrements and saliva of calves and piglets as well as infant faeces. The isolates were identified and a narrower set of lactobacilli and bifidobacteria has been chosen for further testing, where some of their properties having an affinity to adhesion, adherence to human cells, surface hydrophobicity or auto-aggregation were investigated. Positive interplay of all properties important to adhesion provides the benefit of probiotic strains in colonization of the intestine. The most freqeuntly isolated strain was Lactobacillus reuteri and Lactobacillus casei subsp. paracasei. Bifidobacteria were isolated only from calves and piglets (B. thermophilum and B. pseudolongum) and B. longum from calf saliva. Generally, all tested isolates were able to adhere to the tissue model in in vitro conditions. The highest adherence capacity was found in the strain K7 Lacobacillus amylovorus (37,22%). Adherence above 30% was determined primarily in strains isolated from infant faeces (E3TD, E2MD, E3TA and E3M2), also from cow colostrum (K14 and K7).

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů