Two different methods for screening of bile salt hydrolase activity in Lactobacillus strains

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43899624" target="_blank" >RIV/60461373:22330/15:43899624 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.agriculturejournals.cz/web/cjfs.htm?volume=33&type=volume#No" target="_blank" >http://www.agriculturejournals.cz/web/cjfs.htm?volume=33&type=volume#No</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/299/2014-CJFS" target="_blank" >10.17221/299/2014-CJFS</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Two different methods for screening of bile salt hydrolase activity in Lactobacillus strains

Popis výsledku v původním jazyce

Bile salt hydrolase (BSH) activity of intestinal bacteria (including lactobacilli) is one of indirect ways of decreasing cholesterol level at human body. Tested Lactobacillus strains were isolated from various sources (faeces of fully breast-fed infants,cow's colostrum, raw cow's milk cheeses and raw cow's milk) and identified by genotypic and phenotypic methods. All strains, including two commercial probiotic strains and five collection strains, were subsequently tested for their BSH activity via twomethods - Thin Layer Chromatography (TLC) and plate assay. Among all the 45 Lactobacillus strains, 11 strains were shown to be BSH positive by TLC method and only 5 of them by plate assay. Most of the BSH positive strains (7 strains) were isolated from faeces. Differences between BSH activities for sodium salts of cholyltaurine and cholylglycine were demonstrated. TLC method was shown to be more sensitive compared to plate assay for BSH activity determination.

Název v anglickém jazyce

Two different methods for screening of bile salt hydrolase activity in Lactobacillus strains

Popis výsledku anglicky

Bile salt hydrolase (BSH) activity of intestinal bacteria (including lactobacilli) is one of indirect ways of decreasing cholesterol level at human body. Tested Lactobacillus strains were isolated from various sources (faeces of fully breast-fed infants,cow's colostrum, raw cow's milk cheeses and raw cow's milk) and identified by genotypic and phenotypic methods. All strains, including two commercial probiotic strains and five collection strains, were subsequently tested for their BSH activity via twomethods - Thin Layer Chromatography (TLC) and plate assay. Among all the 45 Lactobacillus strains, 11 strains were shown to be BSH positive by TLC method and only 5 of them by plate assay. Most of the BSH positive strains (7 strains) were isolated from faeces. Differences between BSH activities for sodium salts of cholyltaurine and cholylglycine were demonstrated. TLC method was shown to be more sensitive compared to plate assay for BSH activity determination.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Food Sciences

ISSN

1212-1800

e-ISSN

—

Svazek periodika

33

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

13-18

Kód UT WoS článku

000351653300003

EID výsledku v databázi Scopus

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