Identification and characterization of Lacticaseibacillus rhamnosus strains isolated from breast milk and infant faeces

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F22%3AN0000079" target="_blank" >RIV/26722861:_____/22:N0000079 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification and characterization of Lacticaseibacillus rhamnosus strains isolated from breast milk and infant faeces

Popis výsledku v původním jazyce

Lactic acid bacteria, especially genera Lactobacillus and Lacticaseibacillus, are frequently isolated from human gastrointestinal tract, dairy products or breast milk for their remarkable functional and technological properties. In the present study, five strains of Lacticaseibacillus rhamnosus were isolated from infant faeces and breast milk and identified based on their 16S rDNA sequences. Furthermore, isolated strains were tested for aspects of safety, such as antibiotic susceptibility, haemolytic and enzymatic activities. Subsequently, their antimicrobial activity, GIT condition tolerance (low pH, bile salt tolerance), hydrophobicity, antioxidant activity, aggregation ability, and adhesion to Caco-2 and HT-29 were evaluated. Isolates were sensitive to the most of the clinically important antibiotics and none of them showed haemolytic activity. Acidic supernatants of tested strains inhibited the growth of the tested pathogens, such as E.coli, E. faecalis or S. aureus. All isolates were able to survive at pH 2.0 but only two of five Lcb. rhamnosus (21S1E, 21S4F) showed high survival rate in the presence of 3.0 % bile salts (> 80 %), indicating good potential for application as probiotics. Adherence properties varied between strains.Values of hydrophobicity were in the range 12 -50 %, the ability to adhere to Caco2 and HT29 cells made 61-78 % and aggregation between 40-53% after 24 h of cultivation. The results of this characterization indicate that Lacticaseibacillus rhamnosus strains isolated from infant faeces and breast milk have a good potential for use as probiotics in dairy products or functional foods, especially the strain Lcb. rhamnosus 21S1E. Citation: Hyrslova I., Krausova G., Medova K., Drab V., Doskocil I.: Identification and characterization of Lacticaseibacillus rhamnosus strains isolated from breast milk and infant faeces. Ostatní – Poster a sborník abstraktů. Conference: 27th International ICFMH conference FoodMicro 2022, Auqust 28. – 31. 2022, Athens, Greece. In Abstract Book 27th International ICFMH conference FoodMicro 2022, pp. 169.

Název v anglickém jazyce

Identification and characterization of Lacticaseibacillus rhamnosus strains isolated from breast milk and infant faeces

Popis výsledku anglicky

Lactic acid bacteria, especially genera Lactobacillus and Lacticaseibacillus, are frequently isolated from human gastrointestinal tract, dairy products or breast milk for their remarkable functional and technological properties. In the present study, five strains of Lacticaseibacillus rhamnosus were isolated from infant faeces and breast milk and identified based on their 16S rDNA sequences. Furthermore, isolated strains were tested for aspects of safety, such as antibiotic susceptibility, haemolytic and enzymatic activities. Subsequently, their antimicrobial activity, GIT condition tolerance (low pH, bile salt tolerance), hydrophobicity, antioxidant activity, aggregation ability, and adhesion to Caco-2 and HT-29 were evaluated. Isolates were sensitive to the most of the clinically important antibiotics and none of them showed haemolytic activity. Acidic supernatants of tested strains inhibited the growth of the tested pathogens, such as E.coli, E. faecalis or S. aureus. All isolates were able to survive at pH 2.0 but only two of five Lcb. rhamnosus (21S1E, 21S4F) showed high survival rate in the presence of 3.0 % bile salts (> 80 %), indicating good potential for application as probiotics. Adherence properties varied between strains.Values of hydrophobicity were in the range 12 -50 %, the ability to adhere to Caco2 and HT29 cells made 61-78 % and aggregation between 40-53% after 24 h of cultivation. The results of this characterization indicate that Lacticaseibacillus rhamnosus strains isolated from infant faeces and breast milk have a good potential for use as probiotics in dairy products or functional foods, especially the strain Lcb. rhamnosus 21S1E. Citation: Hyrslova I., Krausova G., Medova K., Drab V., Doskocil I.: Identification and characterization of Lacticaseibacillus rhamnosus strains isolated from breast milk and infant faeces. Ostatní – Poster a sborník abstraktů. Conference: 27th International ICFMH conference FoodMicro 2022, Auqust 28. – 31. 2022, Athens, Greece. In Abstract Book 27th International ICFMH conference FoodMicro 2022, pp. 169.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910024" target="_blank" >QK1910024: Využití enzymatického a probiotického potenciálu mikroorganismů k vývoji nových a zvýšení kvality a trvanlivosti stávajících mléčných a pekárenských výrobků.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů