Isolation and identification of three strains of limosilactobacillus reuteri originated from human faeces and their potential as probiotics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F23%3AN0000082" target="_blank" >RIV/26722861:_____/23:N0000082 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation and identification of three strains of limosilactobacillus reuteri originated from human faeces and their potential as probiotics

Popis výsledku v původním jazyce

In the present study, three strains of Limosilactobacillus reuteri isolated from infant faeces were identified based on their 16S rDNA sequences and characterized. Firstly, aspects of safety, such as antibiotic susceptibility, haemolytic and enzymatic activities were determined. Subsequently, their antimicrobial activity against Bacillus cereus, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and Salmonella enterica Typhimurium, hydrophobicity, antioxidant activity, aggregation ability, and adhesion to human adenocarcinoma cell lines Caco-2 and HT-29 were evaluated. Isolates were sensitive to the most of the clinically important antibiotics and none of them showed haemolytic activity and production of harmful enzymes. Acidic supernatants of tested Limosilactobacillus reuteri 21S2A and 21S2C inhibited the growth of all tested pathogens from 80-90 % in contrast to Limosilactobacillus reuteri 21S8A which inhibited pathogens only from 40-50%. The isolates 21S2A and 2C did not show strong hydrophobic properties because the percentages of adhesion to the apolar solvent, n-hexadecane, did not exceed 20 %, showing that their surfaces were rather hydrophilic, on the contrary isolate 21S8A was hydrophobic (67 %). The ability to adhere to Caco2 and HT29 cells made 68-78 % and aggregation about 45 % after 24 h of cultivation. According to presented results, the Limosilactobacillus reuteri strains isolated from infant faeces possess interesting probiotic properties that make them potentially candidates for probiotics. Citation: Hyrslova I., Krausova G., Drab V.: Isolation and identification of three strains of limosilactobacillus reuteri originated from human faeces and their potential as probiotics. Others – Poster. Conference: 4 th International World of Microbiome Conference. Sofia, Bulgaria, 26.–28. October 2023

Název v anglickém jazyce

Isolation and identification of three strains of limosilactobacillus reuteri originated from human faeces and their potential as probiotics

Popis výsledku anglicky

In the present study, three strains of Limosilactobacillus reuteri isolated from infant faeces were identified based on their 16S rDNA sequences and characterized. Firstly, aspects of safety, such as antibiotic susceptibility, haemolytic and enzymatic activities were determined. Subsequently, their antimicrobial activity against Bacillus cereus, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and Salmonella enterica Typhimurium, hydrophobicity, antioxidant activity, aggregation ability, and adhesion to human adenocarcinoma cell lines Caco-2 and HT-29 were evaluated. Isolates were sensitive to the most of the clinically important antibiotics and none of them showed haemolytic activity and production of harmful enzymes. Acidic supernatants of tested Limosilactobacillus reuteri 21S2A and 21S2C inhibited the growth of all tested pathogens from 80-90 % in contrast to Limosilactobacillus reuteri 21S8A which inhibited pathogens only from 40-50%. The isolates 21S2A and 2C did not show strong hydrophobic properties because the percentages of adhesion to the apolar solvent, n-hexadecane, did not exceed 20 %, showing that their surfaces were rather hydrophilic, on the contrary isolate 21S8A was hydrophobic (67 %). The ability to adhere to Caco2 and HT29 cells made 68-78 % and aggregation about 45 % after 24 h of cultivation. According to presented results, the Limosilactobacillus reuteri strains isolated from infant faeces possess interesting probiotic properties that make them potentially candidates for probiotics. Citation: Hyrslova I., Krausova G., Drab V.: Isolation and identification of three strains of limosilactobacillus reuteri originated from human faeces and their potential as probiotics. Others – Poster. Conference: 4 th International World of Microbiome Conference. Sofia, Bulgaria, 26.–28. October 2023

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910024" target="_blank" >QK1910024: Využití enzymatického a probiotického potenciálu mikroorganismů k vývoji nových a zvýšení kvality a trvanlivosti stávajících mléčných a pekárenských výrobků.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů