Enhanced accumulation of Cadmium in Linum ussitatissimum L. plants due to overproduction of metallothionein ?-domain as a fusion to ?-glucuronidase gene protein

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F13%3A%230000793" target="_blank" >RIV/26784246:_____/13:#0000793 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007/s11240-012-0239-1" target="_blank" >http://link.springer.com/article/10.1007/s11240-012-0239-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-012-0239-1" target="_blank" >10.1007/s11240-012-0239-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enhanced accumulation of Cadmium in Linum ussitatissimum L. plants due to overproduction of metallothionein ?-domain as a fusion to ?-glucuronidase gene protein

Popis výsledku v původním jazyce

In an attempt to enhance the phytoextraction capacity of L. usitatissimum through overproduction of an efficient heterologous Cd-binding peptide, we engineered linseed breeding line AGT 917 to constitutively express genetic fusion of ?-domain of mammalian metallothionein 1a (?MT1a) and ?-glucuronidase gus gene under the control of CaMV 35S promoter. An improved transformation protocol was developed. When tested in soils amended with Cd at 20 and 360 mg kg-1, the mature ?MT1a::gus plants accumulated moreCd than parental AGT 917: the stem Cd concentrations in the best performing ?MT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of ?MT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15mg Cd l-1, indicating that ?MT1a::gus did confer higher Cd tolerance to engineered plant. Overproduction of metal-binding peptides thus appears to be a viable strategy for the production of L. usitatissimum with improved phytoremediation

Název v anglickém jazyce

Enhanced accumulation of Cadmium in Linum ussitatissimum L. plants due to overproduction of metallothionein ?-domain as a fusion to ?-glucuronidase gene protein

Popis výsledku anglicky

In an attempt to enhance the phytoextraction capacity of L. usitatissimum through overproduction of an efficient heterologous Cd-binding peptide, we engineered linseed breeding line AGT 917 to constitutively express genetic fusion of ?-domain of mammalian metallothionein 1a (?MT1a) and ?-glucuronidase gus gene under the control of CaMV 35S promoter. An improved transformation protocol was developed. When tested in soils amended with Cd at 20 and 360 mg kg-1, the mature ?MT1a::gus plants accumulated moreCd than parental AGT 917: the stem Cd concentrations in the best performing ?MT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of ?MT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15mg Cd l-1, indicating that ?MT1a::gus did confer higher Cd tolerance to engineered plant. Overproduction of metal-binding peptides thus appears to be a viable strategy for the production of L. usitatissimum with improved phytoremediation

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell Tissue and Organ Culture

ISSN

1573-5044

e-ISSN

—

Svazek periodika

2013

Číslo periodika v rámci svazku

112

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

321-330

Kód UT WoS článku

000315277300006

EID výsledku v databázi Scopus

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