Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F48392952%3A_____%2F13%3A%230000264" target="_blank" >RIV/48392952:_____/13:#0000264 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11240-012-0239-1" target="_blank" >http://dx.doi.org/10.1007/s11240-012-0239-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-012-0239-1" target="_blank" >10.1007/s11240-012-0239-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein

Popis výsledku v původním jazyce

The capacity of most flax (Linum usitatissimum L.) cultivars to accumulate cadmium (Cd) brought an interest in their use in phytoextraction of soil Cd to aboveground biomass. To investigate whether overproduction of an efficient heterologous Cd-binding peptide may enhance the phytoextraction capacity of L. usitatissimum, breeding line AGT 917 was engineered to constitutively express genetic fusion of ?-domain of mammalian metallothionein 1a (?MT1a) and ?-glucuronidase gus gene under the control of CaMV35S promoter. To this end, an improved transformation protocol was developed, which involved 10-min incubation of hypocotyl segments with partially removed epidermis with Agrobacterium suspension in the presence of 100 mg.l-1 acetosyringone and 200 mg.l-1 cellulase and which increased transformation efficiency 1.6-fold as compared to agroinfection without combined acetosyringone and cellulase treatment. When tested in soils amended with Cd added at concentrations of 20 and 360 mg kg-1, t

Název v anglickém jazyce

Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein

Popis výsledku anglicky

The capacity of most flax (Linum usitatissimum L.) cultivars to accumulate cadmium (Cd) brought an interest in their use in phytoextraction of soil Cd to aboveground biomass. To investigate whether overproduction of an efficient heterologous Cd-binding peptide may enhance the phytoextraction capacity of L. usitatissimum, breeding line AGT 917 was engineered to constitutively express genetic fusion of ?-domain of mammalian metallothionein 1a (?MT1a) and ?-glucuronidase gus gene under the control of CaMV35S promoter. To this end, an improved transformation protocol was developed, which involved 10-min incubation of hypocotyl segments with partially removed epidermis with Agrobacterium suspension in the presence of 100 mg.l-1 acetosyringone and 200 mg.l-1 cellulase and which increased transformation efficiency 1.6-fold as compared to agroinfection without combined acetosyringone and cellulase treatment. When tested in soils amended with Cd added at concentrations of 20 and 360 mg kg-1, t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/1M06030" target="_blank" >1M06030: Funkční genomika a proteomika ve šlechtění rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

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Svazek periodika

112

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

321-330

Kód UT WoS článku

000315277300006

EID výsledku v databázi Scopus

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