Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33146005" target="_blank" >RIV/61989592:15310/13:33146005 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/13:43895482

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11240-012-0239-1" target="_blank" >http://dx.doi.org/10.1007/s11240-012-0239-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-012-0239-1" target="_blank" >10.1007/s11240-012-0239-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein.

Popis výsledku v původním jazyce

As most cultivars of flax and linseed (Linum usitatissimum L.) are capable of accumulating cadmium (Cd), they are suitable candidates for phytoextraction of the metal from contaminated soils. In an attempt to enhance the phytoextraction capacity of L. usitatissimum through overproduction of an efficient heterologous Cd-binding peptide, we engineered linseed breeding line AGT 917 to constitutively express genetic fusion of a-domain of mammalian metallothionein 1a (aMT1a) and b-glucuronidase gus gene under the control of CaMV 35S promoter. An improved transformation protocol was developed, which involved cocultivation of AGT 917 hypocotyl segments with partially removed epidermis with Agrobacterium suspension for 10 min in the presence of 200 mg l-1 cellulase.The enzyme treatment increased the transformation efficiency (TE) 1.6- fold as compared to agroinfection without cellulase. Less pronounced impact on TE exerted 100 mg l-1 acetosyringone, increasing TE 1.3-fold. When tested in soils

Název v anglickém jazyce

Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein.

Popis výsledku anglicky

As most cultivars of flax and linseed (Linum usitatissimum L.) are capable of accumulating cadmium (Cd), they are suitable candidates for phytoextraction of the metal from contaminated soils. In an attempt to enhance the phytoextraction capacity of L. usitatissimum through overproduction of an efficient heterologous Cd-binding peptide, we engineered linseed breeding line AGT 917 to constitutively express genetic fusion of a-domain of mammalian metallothionein 1a (aMT1a) and b-glucuronidase gus gene under the control of CaMV 35S promoter. An improved transformation protocol was developed, which involved cocultivation of AGT 917 hypocotyl segments with partially removed epidermis with Agrobacterium suspension for 10 min in the presence of 200 mg l-1 cellulase.The enzyme treatment increased the transformation efficiency (TE) 1.6- fold as compared to agroinfection without cellulase. Less pronounced impact on TE exerted 100 mg l-1 acetosyringone, increasing TE 1.3-fold. When tested in soils

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell Tissue And Organ Culture

ISSN

0167-6857

e-ISSN

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Svazek periodika

112

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

321-330

Kód UT WoS článku

000315277300006

EID výsledku v databázi Scopus

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