Milk acetone determination by the photometrical method after microdiffusion and via FT infra-red spectroscopy

Kód výsledku v IS VaVaI

RIV/26788462:_____/11:#0000531 - isvavai.cz

Nalezeny alternativní kódy

RIV/49608851:_____/11:#0000484

Výsledek na webu

http://www.zf.jcu.cz/obecne/copy_of_dokumenty/dokumenty-journal-of-agrobiology/2011-number-1/Hanus_imprim_web.pdf

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Milk acetone determination by the photometrical method after microdiffusion and via FT infra-red spectroscopy

Popis výsledku v původním jazyce

Milk acetone (AC) is important indicator of energy metabolism of cows. The goal was to investigate infrared method MIR?FT in terms of its calibration for milk AC and to develop an usable procedure. Microdiffusion photometric (485 nm; Spekol 11) method with salicylaldehyde as reference (Re) and mid infrared spectroscopy FT (MIR?FT: Lactoscope FT?IR, Delta; MilkoScan FT 6000, M?Sc) as indirect method were used. The acetone addition to milk had no recovery using MIR?FT (Delta). The reference AC set has tohave an acceptable statistics for good MIR?FT calibration (M?Sc). It was 10.1 ? 9.74 at geometric mean 7.26 mg.l?1, variation range from 1.98 to 33.66 mg.l?1. AC correlation between Re and MIR?FT (Delta) was low 0.32 (P > 0.05). Log AC relationship between Re and MIR?FT (M?Sc) was markedly better 0.80 (P < 0.01). The conversion of > 10 mg.l?1 as AC subclinical ketosis limit was: > ?0.80 (feedback 0.158 mmol.l?1 = 9.25 mg.l?1) and > ?1.66, important for ketosis monitoring by M?Sc.

Název v anglickém jazyce

Milk acetone determination by the photometrical method after microdiffusion and via FT infra-red spectroscopy

Popis výsledku anglicky

Milk acetone (AC) is important indicator of energy metabolism of cows. The goal was to investigate infrared method MIR?FT in terms of its calibration for milk AC and to develop an usable procedure. Microdiffusion photometric (485 nm; Spekol 11) method with salicylaldehyde as reference (Re) and mid infrared spectroscopy FT (MIR?FT: Lactoscope FT?IR, Delta; MilkoScan FT 6000, M?Sc) as indirect method were used. The acetone addition to milk had no recovery using MIR?FT (Delta). The reference AC set has tohave an acceptable statistics for good MIR?FT calibration (M?Sc). It was 10.1 ? 9.74 at geometric mean 7.26 mg.l?1, variation range from 1.98 to 33.66 mg.l?1. AC correlation between Re and MIR?FT (Delta) was low 0.32 (P > 0.05). Log AC relationship between Re and MIR?FT (M?Sc) was markedly better 0.80 (P < 0.01). The conversion of > 10 mg.l?1 as AC subclinical ketosis limit was: > ?0.80 (feedback 0.158 mmol.l?1 = 9.25 mg.l?1) and > ?1.66, important for ketosis monitoring by M?Sc.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

ME09081: Výzkumná a technologická podpora chovu a plemenitby skotu s kombinovanou užitkovostí v provincii Anhui

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Agrobiology

ISSN

1803-4403

e-ISSN

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Svazek periodika

28

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

16

Strana od-do

33-48

Kód UT WoS článku

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EID výsledku v databázi Scopus

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