Molecular modeling and in vivo imaging can identify successful flexible triazine dendrimer-based siRNA delivery systems

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F11%3A43878440" target="_blank" >RIV/44555601:13440/11:43878440 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271103&_user=640945&_pii=S0168365911001015&_check=y&_origin=&_coverDate=15-Jul-2011&view=c&wchp=dGLzVlV-zSkzS&md5=83f002ff8b371e9ba8bb36169e9d3ff8/1-s2.0-S0168365911001015-main.pdf" target="_blank" >http://www.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271103&_user=640945&_pii=S0168365911001015&_check=y&_origin=&_coverDate=15-Jul-2011&view=c&wchp=dGLzVlV-zSkzS&md5=83f002ff8b371e9ba8bb36169e9d3ff8/1-s2.0-S0168365911001015-main.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jconrel.2011.02.016" target="_blank" >10.1016/j.jconrel.2011.02.016</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular modeling and in vivo imaging can identify successful flexible triazine dendrimer-based siRNA delivery systems

Popis výsledku v původním jazyce

This study aimed to identify suitable siRNA delivery systems based on flexible generation 2-4 triazine dendrimers by correlating physico-chemical and biological in vitro and in vivo properties of the complexes with thermodynamic parameters calculated using molecular modeling. The siRNA binding properties of the dendrimers and PEI 25 kDa were simulated, binding and stability were measured in SYBR Gold assays, and hydrodynamic diameters, zeta potentials, and cytotoxicity were quantified. These parameterswere compared with cellular uptake of the complexes and their ability to mediate RNAi. Radiolabeled complexes were administered intravenously, and pharmacokinetic profiles and biodistribution of these polyplexes were assessed both invasively and non-invasively. All flexible triazine dendrimers formed thermodynamically more stable complexes than PEI. While PEI and the generation 4 dendrimer interacted more superficially with siRNA, generation 2 and 3 virtually coalesced with siRNA, formin

Název v anglickém jazyce

Molecular modeling and in vivo imaging can identify successful flexible triazine dendrimer-based siRNA delivery systems

Popis výsledku anglicky

This study aimed to identify suitable siRNA delivery systems based on flexible generation 2-4 triazine dendrimers by correlating physico-chemical and biological in vitro and in vivo properties of the complexes with thermodynamic parameters calculated using molecular modeling. The siRNA binding properties of the dendrimers and PEI 25 kDa were simulated, binding and stability were measured in SYBR Gold assays, and hydrodynamic diameters, zeta potentials, and cytotoxicity were quantified. These parameterswere compared with cellular uptake of the complexes and their ability to mediate RNAi. Radiolabeled complexes were administered intravenously, and pharmacokinetic profiles and biodistribution of these polyplexes were assessed both invasively and non-invasively. All flexible triazine dendrimers formed thermodynamically more stable complexes than PEI. While PEI and the generation 4 dendrimer interacted more superficially with siRNA, generation 2 and 3 virtually coalesced with siRNA, formin

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/OC10053" target="_blank" >OC10053: Dendrimery v biomedicínských aplikacích</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Controlled Release

ISSN

0168-3659

e-ISSN

—

Svazek periodika

153

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

23-33

Kód UT WoS článku

000293433500004

EID výsledku v databázi Scopus

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