Enhancing abdominal wall healing using an oriented polycaprolactone microfibrous scaffold prepared using the fiber drawing method: A rabbit model study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24210%2F26%3A00014392" target="_blank" >RIV/46747885:24210/26:00014392 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24510/26:00014392

Výsledek na webu

<a href="https://doi.org/10.1007/s10029-025-03544-z" target="_blank" >https://doi.org/10.1007/s10029-025-03544-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10029-025-03544-z" target="_blank" >10.1007/s10029-025-03544-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enhancing abdominal wall healing using an oriented polycaprolactone microfibrous scaffold prepared using the fiber drawing method: A rabbit model study

Popis výsledku v původním jazyce

Methods Scaffolds were fabricated from poly(ε-caprolactone) (PCL) using a controlled fiber-drawing technique to produce highly aligned microfibers with reproducible thickness and architecture. Their biocompatibility was examined in vitro using fibroblasts through adhesion and proliferation assays. For in vivo evaluation, the scaffolds were implanted over standardized abdominal wall incisions in rabbits. After six weeks, the regenerated tissue was harvested for mechanical testing to determine tensile strength and elasticity, while histological and immunohistochemical analyses assessed collagen type I deposition and neovascularization within the scaffold area. Results The aligned PCL scaffold promoted strong cell attachment and proliferation in vitro. In vivo, its application significantly increased tensile modulus compared with control wounds. Histological analysis revealed denser and more organized collagen deposition and a higher microvessel density in the scaffold-treated group, indicating enhanced tissue remodeling and vascular integration. Conclusion The aligned PCL microfibrous scaffold improved the mechanical and biological quality of the abdominal wall healing in vivo. These results suggest its potential for reducing the formation of incisional hernias and are suitable for further testing leading to use in clinical practice.

Název v anglickém jazyce

Enhancing abdominal wall healing using an oriented polycaprolactone microfibrous scaffold prepared using the fiber drawing method: A rabbit model study

Popis výsledku anglicky

Methods Scaffolds were fabricated from poly(ε-caprolactone) (PCL) using a controlled fiber-drawing technique to produce highly aligned microfibers with reproducible thickness and architecture. Their biocompatibility was examined in vitro using fibroblasts through adhesion and proliferation assays. For in vivo evaluation, the scaffolds were implanted over standardized abdominal wall incisions in rabbits. After six weeks, the regenerated tissue was harvested for mechanical testing to determine tensile strength and elasticity, while histological and immunohistochemical analyses assessed collagen type I deposition and neovascularization within the scaffold area. Results The aligned PCL scaffold promoted strong cell attachment and proliferation in vitro. In vivo, its application significantly increased tensile modulus compared with control wounds. Histological analysis revealed denser and more organized collagen deposition and a higher microvessel density in the scaffold-treated group, indicating enhanced tissue remodeling and vascular integration. Conclusion The aligned PCL microfibrous scaffold improved the mechanical and biological quality of the abdominal wall healing in vivo. These results suggest its potential for reducing the formation of incisional hernias and are suitable for further testing leading to use in clinical practice.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30212 - Surgery

Projekt

<a href="/cs/project/EH22_008%2F0004562" target="_blank" >EH22_008/0004562: Excelentní výzkum v regenerativní medicíně</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2026

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Hernia>

ISSN

1265-4906

e-ISSN

—

Svazek periodika

30

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

LU - Lucemburské velkovévodství

Počet stran výsledku

20

Strana od-do

—

Kód UT WoS článku

001663663800004

EID výsledku v databázi Scopus

2-s2.0-105027698983