Polycaprolactone Nanofibrous Layer Functionalized by Thrombocyte Rich Solution

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24410%2F13%3A%230001756" target="_blank" >RIV/46747885:24410/13:#0001756 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Polycaprolactone Nanofibrous Layer Functionalized by Thrombocyte Rich Solution

Popis výsledku v původním jazyce

Tissue engineering applications widely use nanofibrous scaffolds due to their similarity to native extracellular matrix. Polycaprolactone (PCL) nanofibers have excellent properties for cell attachment. The platelet growth factors promote cell proliferation. Platelet rich plasma is clinically used in chronic wound treatment and in orthopedic applications (nonunionized fractures, total joint replacement). The aim of presented study is the development of nanofibrous layers functionalized by thrombocytes. The effect of platelet growth factors for fibroblast proliferation was tested. PCL nanofibers were immersed for 2 hours in thrombocyte rich solution (TRS) to enable thrombocyte adhesion. These scaffolds were thereafter seeded with fibroblasts. PCL nanofibrous layers without immobilized thrombocytes were used as a control. The presence of thrombocytes after 2 hour incubation was confirmed by scanning electrone microscopy (SEM). Proliferation of seeded fibroblasts on functionalized scaffold

Název v anglickém jazyce

Polycaprolactone Nanofibrous Layer Functionalized by Thrombocyte Rich Solution

Popis výsledku anglicky

Tissue engineering applications widely use nanofibrous scaffolds due to their similarity to native extracellular matrix. Polycaprolactone (PCL) nanofibers have excellent properties for cell attachment. The platelet growth factors promote cell proliferation. Platelet rich plasma is clinically used in chronic wound treatment and in orthopedic applications (nonunionized fractures, total joint replacement). The aim of presented study is the development of nanofibrous layers functionalized by thrombocytes. The effect of platelet growth factors for fibroblast proliferation was tested. PCL nanofibers were immersed for 2 hours in thrombocyte rich solution (TRS) to enable thrombocyte adhesion. These scaffolds were thereafter seeded with fibroblasts. PCL nanofibrous layers without immobilized thrombocytes were used as a control. The presence of thrombocytes after 2 hour incubation was confirmed by scanning electrone microscopy (SEM). Proliferation of seeded fibroblasts on functionalized scaffold

Druh

O - Ostatní výsledky

CEP obor

JJ - Ostatní materiály

OECD FORD obor

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Projekt

<a href="/cs/project/GAP208%2F12%2F0105" target="_blank" >GAP208/12/0105: ROZTOKY POLYMERŮ VE VNĚJŠÍM POLI: MOLEKULÁRNÍ POCHOPENÍ ELEKTROSPINNINGU</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů