Nanofibrous Polycaprolactone Scaffold with Adhered Platelets Stimulates Proliferation of Skin Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21720%2F16%3A00300671" target="_blank" >RIV/68407700:21720/16:00300671 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/16:00300671

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/cpr.12276/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/cpr.12276/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/cpr.12276" target="_blank" >10.1111/cpr.12276</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nanofibrous Polycaprolactone Scaffold with Adhered Platelets Stimulates Proliferation of Skin Cells

Popis výsledku v původním jazyce

Faulty wound healing is a global healthcare problem. Chronic wounds are generally characterized by a decrease in the availability of growth factors. New strategies are being developed to deliver growth factors more effectively. In this study, we introduced electrospun scaffolds composed of polycaprolactone (PCL) nanofibers functionalized with adhered platelets as a source of numerous growth factors. Different concentrations of platelets were immobilized to nanofibrous scaffolds by simple adhesion and their influence on adhesion, proliferation and metabolic activity of the seeded cells (murine fibroblasts, keratinocytes and melanocytes) was investigated. The data showed the presence of platelets significantly promoted cell spreading, proliferation and metabolic activity in all the skin-associated cell types. There were no significant differences among the tested concentrations of platelets, thus even the lowest tested concentration sufficiently promoted proliferation of the seeded cells. Such complex stimulation is needed for improved healing of chronic wounds. However, the nanofibrous system can be used not only as a skin cover, but for broader applications in regenerative medicine as well.

Název v anglickém jazyce

Nanofibrous Polycaprolactone Scaffold with Adhered Platelets Stimulates Proliferation of Skin Cells

Popis výsledku anglicky

Faulty wound healing is a global healthcare problem. Chronic wounds are generally characterized by a decrease in the availability of growth factors. New strategies are being developed to deliver growth factors more effectively. In this study, we introduced electrospun scaffolds composed of polycaprolactone (PCL) nanofibers functionalized with adhered platelets as a source of numerous growth factors. Different concentrations of platelets were immobilized to nanofibrous scaffolds by simple adhesion and their influence on adhesion, proliferation and metabolic activity of the seeded cells (murine fibroblasts, keratinocytes and melanocytes) was investigated. The data showed the presence of platelets significantly promoted cell spreading, proliferation and metabolic activity in all the skin-associated cell types. There were no significant differences among the tested concentrations of platelets, thus even the lowest tested concentration sufficiently promoted proliferation of the seeded cells. Such complex stimulation is needed for improved healing of chronic wounds. However, the nanofibrous system can be used not only as a skin cover, but for broader applications in regenerative medicine as well.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell Proliferation

ISSN

0960-7722

e-ISSN

—

Svazek periodika

49

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

568-578

Kód UT WoS článku

000383766400004

EID výsledku v databázi Scopus

2-s2.0-84986253488