Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F16%3A00469062" target="_blank" >RIV/68378041:_____/16:00469062 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/16:10327795

Výsledek na webu

<a href="http://dx.doi.org/10.1111/cpr.12276" target="_blank" >http://dx.doi.org/10.1111/cpr.12276</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/cpr.12276" target="_blank" >10.1111/cpr.12276</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells.

Popis výsledku v původním jazyce

ObjectivesFaulty wound healing is a global healthcare problem. Chronic wounds are generally characterized by a reduction in availability of growth factors. New strategies are being developed to deliver growth factors more effectively. nnMethodsIn this study, we introduced electrospun scaffolds composed of polycaprolactone (PCL) nanofibers functionalized with adhered platelets, as a source of numerous growth factors. Three concentrations of platelets were immobilized to nanofibrous scaffolds by simple adhesion, and their influence on adhesion, proliferation and metabolic activity of seeded cells (murine fibroblasts, keratinocytes and melanocytes) was investigated. nnResultsThe data obtained indicated that presence of platelets significantly promoted cell spreading, proliferation and metabolic activity in all the skin-associated cell types. There were no significant differences among tested concentrations of platelets, thus even the lowest concentration sufficiently promoted proliferation of the seeded cells. nnConclusionsSuch complex stimulation is needed for improved healing of chronic wounds. However, the nanofibrous system can be used not only as a skin cover, but also in broader applications in regenerative medicine.

Název v anglickém jazyce

Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells.

Popis výsledku anglicky

ObjectivesFaulty wound healing is a global healthcare problem. Chronic wounds are generally characterized by a reduction in availability of growth factors. New strategies are being developed to deliver growth factors more effectively. nnMethodsIn this study, we introduced electrospun scaffolds composed of polycaprolactone (PCL) nanofibers functionalized with adhered platelets, as a source of numerous growth factors. Three concentrations of platelets were immobilized to nanofibrous scaffolds by simple adhesion, and their influence on adhesion, proliferation and metabolic activity of seeded cells (murine fibroblasts, keratinocytes and melanocytes) was investigated. nnResultsThe data obtained indicated that presence of platelets significantly promoted cell spreading, proliferation and metabolic activity in all the skin-associated cell types. There were no significant differences among tested concentrations of platelets, thus even the lowest concentration sufficiently promoted proliferation of the seeded cells. nnConclusionsSuch complex stimulation is needed for improved healing of chronic wounds. However, the nanofibrous system can be used not only as a skin cover, but also in broader applications in regenerative medicine.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell Proliferation

ISSN

0960-7722

e-ISSN

—

Svazek periodika

49

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

568-578

Kód UT WoS článku

000383766400004

EID výsledku v databázi Scopus

2-s2.0-84986253488