Platelet interaction with nano-and microfibrous materials for the tissue engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24410%2F17%3A00005364" target="_blank" >RIV/46747885:24410/17:00005364 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/27283933:_____/17:00005364

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Platelet interaction with nano-and microfibrous materials for the tissue engineering

Popis výsledku v původním jazyce

Background Tissue engineering is an interdisciplinary field that applies the principles of chemistry, physics, material science, engineering, cell biology and medicine to the development of biological substitutes that restore, maintain or improve tissue/organ functions. Nano‐ and microfibrous materials are essential components for a range of applications in the fields of tissue engineering and regenerative medicine since their morphology resembles significant features of native extracellular matrix. Fibrous scaffolds have a high surface to volume ratio which promotes cell adhesion, migration, proliferation and differentiation. As polymeric fibrous scaffold fabrication techniques strive to create structures that mimics the structure and function of ECM, the need for increased scaffold bioactivity becomes more pronounced. Platelet‐rich plasma (PRP) contain over 300 bioactive molecules. Activated PRP has been shown to contain platelet‐derived growth factor (PDGF), transforming growth factor‐β (TGF‐β), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), epidermal growth factor (EGF) and others in elevated concentrations. PRP has the potential to deliver a combination of GF and cytokines capable of stimulating cellular activity. Incorporation of platelets into scaffold will help to promote scaffold integration within native tissues and increase the overall patency of fibrous structure Aims We used PRP to study interaction between native platelets and fibrous materials with the different morphologies (porosity, fibres diameter – micro, nano). Methods PRP was prepared as whole blood‐derived platelet concentrate (7 × 106 PLT/ml). Fibrous materials were made from polycaprolactone by electrospinning, centrifugal spinning and met‐blown methods. Prepared and sterilized materials were incubated for 2 h with PRP. The degree of platelet adhesion was measured using MTT method. Interaction of PLT with materials was evaluated by fluorescence microscopy (FITC‐anti CD41) and electron microscopy (SEM). The GFs release was monitored by SDS PAGE. Results PLT adhere on both – nano and micro fibrous materials. The adhesion rate of PLT was significantly higher in materials containing nanofibres – either electrospun or in combination with meltblown. SEM pictures shows, that PLT on nanofibres are spread on surface (across more fibres), whereas on microfibrous material they are attached on single fibres. We observed also differences in the aggregation behaviour of platelets on materials with various fibre diameters – PLT form aggregates rather on microfibres than nanofibres. GFs release from PLT is significantly faster when materials contain nanofibres.

Název v anglickém jazyce

Platelet interaction with nano-and microfibrous materials for the tissue engineering

Popis výsledku anglicky

Background Tissue engineering is an interdisciplinary field that applies the principles of chemistry, physics, material science, engineering, cell biology and medicine to the development of biological substitutes that restore, maintain or improve tissue/organ functions. Nano‐ and microfibrous materials are essential components for a range of applications in the fields of tissue engineering and regenerative medicine since their morphology resembles significant features of native extracellular matrix. Fibrous scaffolds have a high surface to volume ratio which promotes cell adhesion, migration, proliferation and differentiation. As polymeric fibrous scaffold fabrication techniques strive to create structures that mimics the structure and function of ECM, the need for increased scaffold bioactivity becomes more pronounced. Platelet‐rich plasma (PRP) contain over 300 bioactive molecules. Activated PRP has been shown to contain platelet‐derived growth factor (PDGF), transforming growth factor‐β (TGF‐β), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), epidermal growth factor (EGF) and others in elevated concentrations. PRP has the potential to deliver a combination of GF and cytokines capable of stimulating cellular activity. Incorporation of platelets into scaffold will help to promote scaffold integration within native tissues and increase the overall patency of fibrous structure Aims We used PRP to study interaction between native platelets and fibrous materials with the different morphologies (porosity, fibres diameter – micro, nano). Methods PRP was prepared as whole blood‐derived platelet concentrate (7 × 106 PLT/ml). Fibrous materials were made from polycaprolactone by electrospinning, centrifugal spinning and met‐blown methods. Prepared and sterilized materials were incubated for 2 h with PRP. The degree of platelet adhesion was measured using MTT method. Interaction of PLT with materials was evaluated by fluorescence microscopy (FITC‐anti CD41) and electron microscopy (SEM). The GFs release was monitored by SDS PAGE. Results PLT adhere on both – nano and micro fibrous materials. The adhesion rate of PLT was significantly higher in materials containing nanofibres – either electrospun or in combination with meltblown. SEM pictures shows, that PLT on nanofibres are spread on surface (across more fibres), whereas on microfibrous material they are attached on single fibres. We observed also differences in the aggregation behaviour of platelets on materials with various fibre diameters – PLT form aggregates rather on microfibres than nanofibres. GFs release from PLT is significantly faster when materials contain nanofibres.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30230 - Other clinical medicine subjects

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů