Fabrication by laser irradiation in a continuous flow jet of carbon quantum dots for fluorescence imaging

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F18%3A00004948" target="_blank" >RIV/46747885:24620/18:00004948 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044845/pdf/ao7b02082.pdf" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044845/pdf/ao7b02082.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsomega.7b02082" target="_blank" >10.1021/acsomega.7b02082</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fabrication by laser irradiation in a continuous flow jet of carbon quantum dots for fluorescence imaging

Popis výsledku v původním jazyce

Fluorescent carbon quantum dots (CQDs) are synthesized by laser irradiation of carbon glassy suspended in polyethylene glycol 200 by two methods, a batch and a flow jet configuration. The flow jet is done by simple combination of common laboratory objects to construct a home-made passage reactor of continuous flow. Despite the simplicity of the system, the laser energy is better harvested by the carbon microparticles improving the fabrication efficiency a 15% and enhancing fluorescence of CQDs by an order of magnitude in comparison with the conventional batch. The flow jet synthesized CQDs have a mean size of 3 nm and are used for fluorescent imaging of transparent healthy and cancer epithelial human cells. Complete internalization is observed with a short incubation time of 10 minutes without using any extra additive or processing of the cell culture. The CQDs are well fixed in the organelles of the cell even after its death, so this is a simple manner to keep the cell information for prolonged periods of time. Moreover, the integrated photostability of the CQDs internalized in in vitro cells is measured and it keeps almost constant during at least 2 hours revealing their outstanding perform as fluorescent labels.

Název v anglickém jazyce

Fabrication by laser irradiation in a continuous flow jet of carbon quantum dots for fluorescence imaging

Popis výsledku anglicky

Fluorescent carbon quantum dots (CQDs) are synthesized by laser irradiation of carbon glassy suspended in polyethylene glycol 200 by two methods, a batch and a flow jet configuration. The flow jet is done by simple combination of common laboratory objects to construct a home-made passage reactor of continuous flow. Despite the simplicity of the system, the laser energy is better harvested by the carbon microparticles improving the fabrication efficiency a 15% and enhancing fluorescence of CQDs by an order of magnitude in comparison with the conventional batch. The flow jet synthesized CQDs have a mean size of 3 nm and are used for fluorescent imaging of transparent healthy and cancer epithelial human cells. Complete internalization is observed with a short incubation time of 10 minutes without using any extra additive or processing of the cell culture. The CQDs are well fixed in the organelles of the cell even after its death, so this is a simple manner to keep the cell information for prolonged periods of time. Moreover, the integrated photostability of the CQDs internalized in in vitro cells is measured and it keeps almost constant during at least 2 hours revealing their outstanding perform as fluorescent labels.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

<a href="/cs/project/LO1201" target="_blank" >LO1201: ROZVOJ ÚSTAVU PRO NANOMATERIÁLY, POKROČILÉ TECHNOLOGIE A INOVACE TECHNICKÉ UNIVERZITY V LIBERCI</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Omega

ISSN

2470-1343

e-ISSN

—

Svazek periodika

3

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

2735-2742

Kód UT WoS článku

000427939400030

EID výsledku v databázi Scopus

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