Mucoadhesive nanomaterials - Composition and size matters

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F23%3A00011596" target="_blank" >RIV/46747885:24620/23:00011596 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Mucoadhesive nanomaterials - Composition and size matters

Popis výsledku v původním jazyce

Abstract presented at international conference Termis EU 2023 in Mancheter, UK, March 28-31. Meeting abstracts published in Tissue engineering Part A, Vol. 29, Issue 13-14, 2023. Abstract: INTRODUCTION:Nano-based drug dosage forms have attracted attention of pharmaceutical industry due to their unique properties and ability to overcome various physiological barriers and surfaces. Among all, the mucosal surface plays a crucial role for in situ sublingual, eye, vaginal and intestinal delivery of large number of drugs. Administration through these tissues is a challenge due to the poor mucoadhesion of the current formulations often leading to very low DDS stability and therefore insufficient bioavailability of delivered drug. For example, 90% of the drugs used in ophthalmology are administered with ocular drops onto the mucosal layer of the cornea, due to poor mucoadhesion of DDS formulation and only 0.0006% to 0.02% of the active molecules administered onto the cornea are able to reach the anterior chamber of the eye, where most of intended drug targets are located [1]. From the nutrition point of view, interactions with intestinal mucosal barrier are crucial for biomolecules reception. The development of efficient mucoadhesive drug delivery systems (DDS) is thus a crucial task to improve bioavailability, stability and therapeutic performance of drugs delivered in situ on various mucosal tissues. In nanomaterials, the degree of mucoadhesion depends on several aspects – dimension, chemical composition, surface charge and wettability. METHODS:In our work, several types of nanomaterials in form of nanoparticles and nanofibres based on PCL, chitosan, silica and CeO2 were studied on mucoadhesion in vitro. Size and surface morphology was evaluated using SEM and DLS. The material-mucin interactions were tested by several methods under simulated physiological conditions. Moreover, concentration dependent cytotoxicity of nanomaterials to intestinal epithelium model based on Caco-2 cells and corneal epithelium model based on HCE cells was studied in vitro. RESULTS:The nanomaterial-mucin interactions were proven to be composition and size dependent with the best results obtained from chitosan nanomaterials. In the case of PCL nanofibres, strong impact of fibre morphology was observed with amount of bound mucin growing with decreasing fibre diameter. DISCUSSION & CONCLUSIONS:Increasing specific surface leads to improved mucin adhesion to nanomaterials. Chitosan nanomaterials represent highly mucoadhesive DDS due to their positive charge and strong interactions with mucin.

Název v anglickém jazyce

Mucoadhesive nanomaterials - Composition and size matters

Popis výsledku anglicky

Abstract presented at international conference Termis EU 2023 in Mancheter, UK, March 28-31. Meeting abstracts published in Tissue engineering Part A, Vol. 29, Issue 13-14, 2023. Abstract: INTRODUCTION:Nano-based drug dosage forms have attracted attention of pharmaceutical industry due to their unique properties and ability to overcome various physiological barriers and surfaces. Among all, the mucosal surface plays a crucial role for in situ sublingual, eye, vaginal and intestinal delivery of large number of drugs. Administration through these tissues is a challenge due to the poor mucoadhesion of the current formulations often leading to very low DDS stability and therefore insufficient bioavailability of delivered drug. For example, 90% of the drugs used in ophthalmology are administered with ocular drops onto the mucosal layer of the cornea, due to poor mucoadhesion of DDS formulation and only 0.0006% to 0.02% of the active molecules administered onto the cornea are able to reach the anterior chamber of the eye, where most of intended drug targets are located [1]. From the nutrition point of view, interactions with intestinal mucosal barrier are crucial for biomolecules reception. The development of efficient mucoadhesive drug delivery systems (DDS) is thus a crucial task to improve bioavailability, stability and therapeutic performance of drugs delivered in situ on various mucosal tissues. In nanomaterials, the degree of mucoadhesion depends on several aspects – dimension, chemical composition, surface charge and wettability. METHODS:In our work, several types of nanomaterials in form of nanoparticles and nanofibres based on PCL, chitosan, silica and CeO2 were studied on mucoadhesion in vitro. Size and surface morphology was evaluated using SEM and DLS. The material-mucin interactions were tested by several methods under simulated physiological conditions. Moreover, concentration dependent cytotoxicity of nanomaterials to intestinal epithelium model based on Caco-2 cells and corneal epithelium model based on HCE cells was studied in vitro. RESULTS:The nanomaterial-mucin interactions were proven to be composition and size dependent with the best results obtained from chitosan nanomaterials. In the case of PCL nanofibres, strong impact of fibre morphology was observed with amount of bound mucin growing with decreasing fibre diameter. DISCUSSION & CONCLUSIONS:Increasing specific surface leads to improved mucin adhesion to nanomaterials. Chitosan nanomaterials represent highly mucoadhesive DDS due to their positive charge and strong interactions with mucin.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů