Typing of MRSA strains using different molecular-genetic methods
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49608851%3A_____%2F11%3A%230000510" target="_blank" >RIV/49608851:_____/11:#0000510 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Typing of MRSA strains using different molecular-genetic methods
Popis výsledku v původním jazyce
The aim of our work was to apply different molecular techniques to type MRSA strains, compare acquired results and performance of these methods. The collection of eight MRSA strains from different livestock farms in the Czech Republic was analysed. To type the strains, the MLST (Multi Locus Sequence typing), spa and SCCmec (Staphylococcal Chromosome Cassette mec) typing, rep-PCR analyse, ribotyping and PFGE (Pulsed-field gel electrophoresis) were applied. The (GTG)5 primer and the GelCompare software were used for the rep-PCR analyse. The ribotyping of the strains was performed on the RiboPrinterTM system, the SmaI restriction endonuclease was applied for the PFGE analyse. The SCCmec typing revealed presence of 4, 4D and 5 SCCmec type, the MLST analyseidentified the ST8 and ST398 type among tested strains. Analysis of spa profiles revealed presence of five different strains. Also the rep-PCR indicated presence of five types of strains, however with distinct distribution. The ribotypin
Název v anglickém jazyce
Typing of MRSA strains using different molecular-genetic methods
Popis výsledku anglicky
The aim of our work was to apply different molecular techniques to type MRSA strains, compare acquired results and performance of these methods. The collection of eight MRSA strains from different livestock farms in the Czech Republic was analysed. To type the strains, the MLST (Multi Locus Sequence typing), spa and SCCmec (Staphylococcal Chromosome Cassette mec) typing, rep-PCR analyse, ribotyping and PFGE (Pulsed-field gel electrophoresis) were applied. The (GTG)5 primer and the GelCompare software were used for the rep-PCR analyse. The ribotyping of the strains was performed on the RiboPrinterTM system, the SmaI restriction endonuclease was applied for the PFGE analyse. The SCCmec typing revealed presence of 4, 4D and 5 SCCmec type, the MLST analyseidentified the ST8 and ST398 type among tested strains. Analysis of spa profiles revealed presence of five different strains. Also the rep-PCR indicated presence of five types of strains, however with distinct distribution. The ribotypin
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů