Molecular characterization of MRSA strains isolated from livestock milk and meat in the Czech Republic

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular characterization of MRSA strains isolated from livestock milk and meat in the Czech Republic

Popis výsledku v původním jazyce

Aims: The livestock milk and meat industry represent a possible source of MRSA strains, with potential to infect the humans or infiltrate the food chain. The aim of the study was to apply different molecular genetic methods to type acquired MRSA strainsMethods and results: Hundreds of samples of cow, goat and sheep milk, pork meat and farm personnel were collected in two years in the Czech Republic. Eight MRSA strains from different farms were purchased and analysed using MLST (Multi Locus Sequence typing), spa and SCCmec (Staphylococcal Chromosome Cassette mec) typing based on PCR. The (GTG)5 primer was applied for the rep-PCR analyse. The ribotypization of the strains was performed on the RiboPrinterTM system. The individual strains possessed resistance to 3-8 different antibiotics, two isolates carried the gene seb encoding enterotoxin B. The strains belonged to the 4, 4D and 5 SCCmec type; t011, t034 and t064 spa type and ST8, ST398 MLST type. The results of ribotypization reveale

Název v anglickém jazyce

Molecular characterization of MRSA strains isolated from livestock milk and meat in the Czech Republic

Popis výsledku anglicky

Aims: The livestock milk and meat industry represent a possible source of MRSA strains, with potential to infect the humans or infiltrate the food chain. The aim of the study was to apply different molecular genetic methods to type acquired MRSA strainsMethods and results: Hundreds of samples of cow, goat and sheep milk, pork meat and farm personnel were collected in two years in the Czech Republic. Eight MRSA strains from different farms were purchased and analysed using MLST (Multi Locus Sequence typing), spa and SCCmec (Staphylococcal Chromosome Cassette mec) typing based on PCR. The (GTG)5 primer was applied for the rep-PCR analyse. The ribotypization of the strains was performed on the RiboPrinterTM system. The individual strains possessed resistance to 3-8 different antibiotics, two isolates carried the gene seb encoding enterotoxin B. The strains belonged to the 4, 4D and 5 SCCmec type; t011, t034 and t064 spa type and ST8, ST398 MLST type. The results of ribotypization reveale

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů