Determination of biogenic amines in foods using ultra-performance liquid chromatography (UPLC)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F09%3A00010865" target="_blank" >RIV/60076658:12220/09:00010865 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of biogenic amines in foods using ultra-performance liquid chromatography (UPLC)

Popis výsledku v původním jazyce

A rapid ultra-performance liquid chromatographic (UPLC) method for the determination of biogenic amines (putrescine, cadaverine, spermidine, spermine, phenylethylamine, histamine, tyramine and tryptamine) in selected food samples is described. The 8 biogenic amines, which are the most important to be determined in food samples, were derivatized with dansyl chloride prior to UPLC separation. The dansylated amines were separated on an Agilent Zorbax Eclipse XDB ? C18 column (50 x 4,6 mm I.D., 1.8 m) usinggradient elution with a binary system of acetonitrile-water, a flow-rate of 1.0 ml/min and UV detection at 225 nm. The average repeatability of the method ranged between 1.02 ? 2.14 %. Limits of detection (LODs) ranged between 0.032 ? 0.098 g/l; calibration curves showed very good linearity (r = 0.9994 ? 1.0000). The method was applied to the analysis of amines in pork, beef, chicken and fish meat, cheese and edible mushrooms.

Název v anglickém jazyce

Determination of biogenic amines in foods using ultra-performance liquid chromatography (UPLC)

Popis výsledku anglicky

A rapid ultra-performance liquid chromatographic (UPLC) method for the determination of biogenic amines (putrescine, cadaverine, spermidine, spermine, phenylethylamine, histamine, tyramine and tryptamine) in selected food samples is described. The 8 biogenic amines, which are the most important to be determined in food samples, were derivatized with dansyl chloride prior to UPLC separation. The dansylated amines were separated on an Agilent Zorbax Eclipse XDB ? C18 column (50 x 4,6 mm I.D., 1.8 m) usinggradient elution with a binary system of acetonitrile-water, a flow-rate of 1.0 ml/min and UV detection at 225 nm. The average repeatability of the method ranged between 1.02 ? 2.14 %. Limits of detection (LODs) ranged between 0.032 ? 0.098 g/l; calibration curves showed very good linearity (r = 0.9994 ? 1.0000). The method was applied to the analysis of amines in pork, beef, chicken and fish meat, cheese and edible mushrooms.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Chemistry

ISSN

0308-8146

e-ISSN

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Svazek periodika

116

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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