The assessment of thermostable proteins gained from different potato tuber cultivars (Solanum tuberosum L.) and their influence on growth inhibition of selected phytopathogenic fungi

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F13%3A43886342" target="_blank" >RIV/60076658:12220/13:43886342 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The assessment of thermostable proteins gained from different potato tuber cultivars (Solanum tuberosum L.) and their influence on growth inhibition of selected phytopathogenic fungi

Popis výsledku v původním jazyce

The contribution deals with the assessments of antifungal potential of thermostable proteins from three potato cultivars (Solanum tuberosum L.) David, Marabel and Westamyl differ in the market and maturity and comparison of two potato fruit juice alternatives differing in processing temperature. Combination of pH adjustment and high temperature (80 °C) or pH adjustment only was used for protein isolation. Isolated proteins were detected by peptide SDS-PAGE in range from 6 to 14 kDa and in ranges 14 and17 kDa in the sample of the thermally untreated potato fruit juice. Three different concentrations of protein samples were applied to three different varieties of phytopathogenic fungi Alternaria solani, Fusarium solani and Rhizoctonia solani. The inhibition of mycelial growth was evaluated after 24 hours of growth at 16 °C on digital photos by special software using the method of measuring the inhibition area. The greatest inhibition area (108,9 mm2) was achieved on F. solani cultivar D

Název v anglickém jazyce

The assessment of thermostable proteins gained from different potato tuber cultivars (Solanum tuberosum L.) and their influence on growth inhibition of selected phytopathogenic fungi

Popis výsledku anglicky

The contribution deals with the assessments of antifungal potential of thermostable proteins from three potato cultivars (Solanum tuberosum L.) David, Marabel and Westamyl differ in the market and maturity and comparison of two potato fruit juice alternatives differing in processing temperature. Combination of pH adjustment and high temperature (80 °C) or pH adjustment only was used for protein isolation. Isolated proteins were detected by peptide SDS-PAGE in range from 6 to 14 kDa and in ranges 14 and17 kDa in the sample of the thermally untreated potato fruit juice. Three different concentrations of protein samples were applied to three different varieties of phytopathogenic fungi Alternaria solani, Fusarium solani and Rhizoctonia solani. The inhibition of mycelial growth was evaluated after 24 hours of growth at 16 °C on digital photos by special software using the method of measuring the inhibition area. The greatest inhibition area (108,9 mm2) was achieved on F. solani cultivar D

Druh

D - Stať ve sborníku

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F09%2F1693" target="_blank" >GA522/09/1693: Úloha antimikrobiálních peptidů v obraně rostlin proti pathogenům</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 2nd International Symposium on Agronomy and Physiology of Potato

ISBN

978-80-86940-52-6

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

24-29

Název nakladatele

Tisk Hermann spol.

Místo vydání

Potato Research Institute Havlíčkův Brod

Místo konání akce

Praha

Datum konání akce

15. 9. 2013

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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