Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F15%3A43887835" target="_blank" >RIV/60076658:12220/15:43887835 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/15:00443160

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10658-014-0554-y" target="_blank" >http://dx.doi.org/10.1007/s10658-014-0554-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10658-014-0554-y" target="_blank" >10.1007/s10658-014-0554-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria

Popis výsledku v původním jazyce

We tested 212 bacterial isolates isolated from tomato and pepper plants suspected of being infected by Xanthomonas vesicatoria (causing bacterial spot). Samples from plants were collected in the years 2007- 2010 from fields located in central Bohemia andcentral and south Moravia. For detection of the pathogens we used PCR with two previously published primer pairs (RST65, RST69 and XCVF, XCVR) and one primer pair (Xv1, Xv2) designed for specific detection of Xanthomonas vesicatoria. In total, 121 of 212 (57%) samples were positive in PCR. In this article we provide data for additional verification of the newly designed (Xv1, Xv2) primers. Our results confirm the need of precise detection methods, because several tomato and pepper pathogens can share similar visual symptoms. We also suggest, that new PCR primers for detection of bacterial spot of tomato and pepper pathogens should not be overlooked, because they can improve currently used PCR assays for detection of these pathogens.

Název v anglickém jazyce

Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria

Popis výsledku anglicky

We tested 212 bacterial isolates isolated from tomato and pepper plants suspected of being infected by Xanthomonas vesicatoria (causing bacterial spot). Samples from plants were collected in the years 2007- 2010 from fields located in central Bohemia andcentral and south Moravia. For detection of the pathogens we used PCR with two previously published primer pairs (RST65, RST69 and XCVF, XCVR) and one primer pair (Xv1, Xv2) designed for specific detection of Xanthomonas vesicatoria. In total, 121 of 212 (57%) samples were positive in PCR. In this article we provide data for additional verification of the newly designed (Xv1, Xv2) primers. Our results confirm the need of precise detection methods, because several tomato and pepper pathogens can share similar visual symptoms. We also suggest, that new PCR primers for detection of bacterial spot of tomato and pepper pathogens should not be overlooked, because they can improve currently used PCR assays for detection of these pathogens.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Plant Pathology

ISSN

0929-1873

e-ISSN

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Svazek periodika

2015

Číslo periodika v rámci svazku

141

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

617-621

Kód UT WoS článku

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EID výsledku v databázi Scopus

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