Development of loop mediated isothermal amplification for rapid species detection of Armillaria ostoyae using assimilating probe.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F22%3A43905868" target="_blank" >RIV/60076658:12220/22:43905868 - isvavai.cz</a>

Výsledek na webu

<a href="https://jfs.agriculturejournals.cz/artkey/jfs-202205-0001_development-of-loop-mediated-isothermal-amplification-for-rapid-species-detection-of-armillaria-ostoyae-using-a.php" target="_blank" >https://jfs.agriculturejournals.cz/artkey/jfs-202205-0001_development-of-loop-mediated-isothermal-amplification-for-rapid-species-detection-of-armillaria-ostoyae-using-a.php</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/161/2021-JFS" target="_blank" >10.17221/161/2021-JFS</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of loop mediated isothermal amplification for rapid species detection of Armillaria ostoyae using assimilating probe.

Popis výsledku v původním jazyce

We introduced here the first loop mediated isothermal amplification (LAMP) assay for the identification of honey fungus, Armillaria ostoyae, a basidiomycote playing an important role in spruce declines in the Palaearctic region. In total, 101 isolates, representing three Armillaria species, were used to develop a new LAMP assay to determine species specific identification. We have here described LAMP primers enhanced with fluorescent dye that are able to amplify A. ostoyae DNA and detect fungi in a fast single step reaction. The detection limit of LAMP was 1 pg of genomic DNA per reaction. We optimized a new LAMP assay for the rapid detection of A. ostoyae using the translation elongation factor 1-α (tef1) marker and fluorescence labelled oligonucleotide assimilating probe. The LAMP assay does not require any specialized equipment, hence it can be used in the field for the rapid detection of A. ostoyae even using the portable and mobile device. The specificity of the assay was confirmed by the use of A. ostoyae strains and Armillaria cepistipes and Armillaria gallica strains, respectively. In conclusion, the assay could be a rapid, specific, sensitive and low-cost tool for identification of A. ostoyae as well as the first step for expansion of this method in practical applications.

Název v anglickém jazyce

Development of loop mediated isothermal amplification for rapid species detection of Armillaria ostoyae using assimilating probe.

Popis výsledku anglicky

We introduced here the first loop mediated isothermal amplification (LAMP) assay for the identification of honey fungus, Armillaria ostoyae, a basidiomycote playing an important role in spruce declines in the Palaearctic region. In total, 101 isolates, representing three Armillaria species, were used to develop a new LAMP assay to determine species specific identification. We have here described LAMP primers enhanced with fluorescent dye that are able to amplify A. ostoyae DNA and detect fungi in a fast single step reaction. The detection limit of LAMP was 1 pg of genomic DNA per reaction. We optimized a new LAMP assay for the rapid detection of A. ostoyae using the translation elongation factor 1-α (tef1) marker and fluorescence labelled oligonucleotide assimilating probe. The LAMP assay does not require any specialized equipment, hence it can be used in the field for the rapid detection of A. ostoyae even using the portable and mobile device. The specificity of the assay was confirmed by the use of A. ostoyae strains and Armillaria cepistipes and Armillaria gallica strains, respectively. In conclusion, the assay could be a rapid, specific, sensitive and low-cost tool for identification of A. ostoyae as well as the first step for expansion of this method in practical applications.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

—

OECD FORD obor

10612 - Mycology

Projekt

<a href="/cs/project/QK1920412" target="_blank" >QK1920412: Mykoviry jako součást potenciálních biopreparátů v ochraně smrkových porostů proti václavkám</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Forest Science

ISSN

1212-4834

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

163-169

Kód UT WoS článku

000800595600001

EID výsledku v databázi Scopus

2-s2.0-85131895605