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Between sexual and apomictic: unexpectedly variable sporogenesis and production of viable polyhaploids in the pentaploid fern of the Dryopteris affinis agg. (Dryopteridaceae)

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F16%3A43890568" target="_blank" >RIV/60076658:12310/16:43890568 - isvavai.cz</a>

  • Výsledek na webu

    <a href="http://aob.oxfordjournals.org/content/117/1/97" target="_blank" >http://aob.oxfordjournals.org/content/117/1/97</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1093/aob/mcv152" target="_blank" >10.1093/aob/mcv152</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Between sexual and apomictic: unexpectedly variable sporogenesis and production of viable polyhaploids in the pentaploid fern of the Dryopteris affinis agg. (Dryopteridaceae)

  • Popis výsledku v původním jazyce

    Background and Aims In ferns, apomixis is an important mode of asexual reproduction. Although the mechanisms of fern reproduction have been studied thoroughly, most previous work has focused on cases in which ferns reproduce either exclusively sexually or exclusively asexually. Reproduction of ferns with potentially mixed systems and inheritance of apomixis remains largely unknown. This study addresses reproduction of the pentaploid Dryopteris x critica, a hybrid of triploid apomictic D. borreri and tetraploid sexual D. filix-mas. Methods Spore size, abortion percentage and number of spores per sporangium were examined in pentaploid plants of D. x critica grown in an experimental garden. The sporangial content of leaf segments was cultivated on an agar medium, and DNA ploidy levels were estimated by DAPI flow cytometry in 259 gametophytes or sporophytes arising from the F-2 generation of the pentaploid hybrid. Key Results The hybrid is partly fertile (89-94% of aborted spores) and shows unstable sporogenesis with sexual and apomictic reproduction combined. The number of spores per sporangium varied from approx. 31 to 64. Within a single sporangium it was possible to detect formation of either only aborted spores or various mixtures of aborted and well-developed reduced spores and unreduced diplospores. The spores germinated in viable gametophytes with two ploidy levels: pentaploid (5x, from unreduced spores) and half of that (approx. 2.5x, from reduced spores). Moreover, 2-15% of gametophytes (both 2.5x and 5x) formed a viable sporophyte of the same ploidy level due to apogamy. Conclusions This study documents the mixed reproductive mode of a hybrid between apomictic and sexual ferns. Both sexual reduced and apomictic unreduced spores can be produced by a single individual, and even within a single sporangium. Both types of spores give rise to viable F-2 generation gametophytes and sporophytes.

  • Název v anglickém jazyce

    Between sexual and apomictic: unexpectedly variable sporogenesis and production of viable polyhaploids in the pentaploid fern of the Dryopteris affinis agg. (Dryopteridaceae)

  • Popis výsledku anglicky

    Background and Aims In ferns, apomixis is an important mode of asexual reproduction. Although the mechanisms of fern reproduction have been studied thoroughly, most previous work has focused on cases in which ferns reproduce either exclusively sexually or exclusively asexually. Reproduction of ferns with potentially mixed systems and inheritance of apomixis remains largely unknown. This study addresses reproduction of the pentaploid Dryopteris x critica, a hybrid of triploid apomictic D. borreri and tetraploid sexual D. filix-mas. Methods Spore size, abortion percentage and number of spores per sporangium were examined in pentaploid plants of D. x critica grown in an experimental garden. The sporangial content of leaf segments was cultivated on an agar medium, and DNA ploidy levels were estimated by DAPI flow cytometry in 259 gametophytes or sporophytes arising from the F-2 generation of the pentaploid hybrid. Key Results The hybrid is partly fertile (89-94% of aborted spores) and shows unstable sporogenesis with sexual and apomictic reproduction combined. The number of spores per sporangium varied from approx. 31 to 64. Within a single sporangium it was possible to detect formation of either only aborted spores or various mixtures of aborted and well-developed reduced spores and unreduced diplospores. The spores germinated in viable gametophytes with two ploidy levels: pentaploid (5x, from unreduced spores) and half of that (approx. 2.5x, from reduced spores). Moreover, 2-15% of gametophytes (both 2.5x and 5x) formed a viable sporophyte of the same ploidy level due to apogamy. Conclusions This study documents the mixed reproductive mode of a hybrid between apomictic and sexual ferns. Both sexual reduced and apomictic unreduced spores can be produced by a single individual, and even within a single sporangium. Both types of spores give rise to viable F-2 generation gametophytes and sporophytes.

Klasifikace

  • Druh

    J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

  • CEP obor

    EH - Ekologie – společenstva

  • OECD FORD obor

Návaznosti výsledku

  • Projekt

    <a href="/cs/project/GB14-36079G" target="_blank" >GB14-36079G: Centrum analýzy a syntézy rostlinné diverzity (PLADIAS)</a><br>

  • Návaznosti

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Ostatní

  • Rok uplatnění

    2016

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Annals of Botany

  • ISSN

    0305-7364

  • e-ISSN

  • Svazek periodika

    117

  • Číslo periodika v rámci svazku

    1

  • Stát vydavatele periodika

    GB - Spojené království Velké Británie a Severního Irska

  • Počet stran výsledku

    10

  • Strana od-do

    97-106

  • Kód UT WoS článku

    000369996700008

  • EID výsledku v databázi Scopus