An adventurous journey toward and away from fern apomixis: Insights from genome size and spore abortion patterns

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F24%3A43908000" target="_blank" >RIV/60076658:12310/24:43908000 - isvavai.cz</a>

Výsledek na webu

<a href="https://bsapubs.onlinelibrary.wiley.com/doi/10.1002/ajb2.16332" target="_blank" >https://bsapubs.onlinelibrary.wiley.com/doi/10.1002/ajb2.16332</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/ajb2.16332" target="_blank" >10.1002/ajb2.16332</a>

Jazyk výsledku

angličtina

Název v původním jazyce

An adventurous journey toward and away from fern apomixis: Insights from genome size and spore abortion patterns

Popis výsledku v původním jazyce

PremiseApomixis in ferns is relatively common and obligatory. Sterile hybrids may restore fertility via apomixis at a cost of long-term genetic stagnation. In this study, we outlined apomixis as a possible temporary phase leading to sexuality and analyzed factors relating to transitioning to and away from apomixis, such as unreduced and reduced spore formation in apomict and apo-sex hybrid ferns.MethodsWe analyzed the genome size of 15 fern species or hybrids (&quot;taxa&quot;) via flow cytometry. The number of reduced and unreduced gametophytes was established as a proxy for viable spore formation of either type. We also calculated the spore abortion ratio (sign of reduced spores) in several taxa, including the apo-sex hybrid Dryopteris x critica and its 16 apomictically formed offspring.ResultsFour of 15 sampled taxa yielded offspring variable in genome size. Specifically, each variable taxon formed one viable reduced plant among 12-451 sampled gametophytes per taxon. Thus, haploid spore formation in the studied apomicts was very rare but possible. Spore abortion analyses indicated gradually decreasing abortion (haploid spore formation) over time. In Dryopteris x critica, abortion decreased from 93.8% to mean 89.5% in one generation.ConclusionsOur results support apomixis as a transitionary phase toward sexuality. Newly formed apomicts hybridize with sexual relatives and continue to form haploid spores early on. Thus, they may get the genomic content necessary for regular meiosis and restore sexuality. If the missing relative goes extinct, the lineage gets locked into apomixis as may be the case with the Dryopteris affinis complex.

Název v anglickém jazyce

An adventurous journey toward and away from fern apomixis: Insights from genome size and spore abortion patterns

Popis výsledku anglicky

PremiseApomixis in ferns is relatively common and obligatory. Sterile hybrids may restore fertility via apomixis at a cost of long-term genetic stagnation. In this study, we outlined apomixis as a possible temporary phase leading to sexuality and analyzed factors relating to transitioning to and away from apomixis, such as unreduced and reduced spore formation in apomict and apo-sex hybrid ferns.MethodsWe analyzed the genome size of 15 fern species or hybrids (&quot;taxa&quot;) via flow cytometry. The number of reduced and unreduced gametophytes was established as a proxy for viable spore formation of either type. We also calculated the spore abortion ratio (sign of reduced spores) in several taxa, including the apo-sex hybrid Dryopteris x critica and its 16 apomictically formed offspring.ResultsFour of 15 sampled taxa yielded offspring variable in genome size. Specifically, each variable taxon formed one viable reduced plant among 12-451 sampled gametophytes per taxon. Thus, haploid spore formation in the studied apomicts was very rare but possible. Spore abortion analyses indicated gradually decreasing abortion (haploid spore formation) over time. In Dryopteris x critica, abortion decreased from 93.8% to mean 89.5% in one generation.ConclusionsOur results support apomixis as a transitionary phase toward sexuality. Newly formed apomicts hybridize with sexual relatives and continue to form haploid spores early on. Thus, they may get the genomic content necessary for regular meiosis and restore sexuality. If the missing relative goes extinct, the lineage gets locked into apomixis as may be the case with the Dryopteris affinis complex.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/GA19-17379S" target="_blank" >GA19-17379S: Boj o nadvládu mezi sexuály a apomikty: mechanismy a následky konfliktu mezi dvěma typy reprodukce u kapraďů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

American Journal of Botany

ISSN

0002-9122

e-ISSN

—

Svazek periodika

111

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

—

Kód UT WoS článku

001225644800001

EID výsledku v databázi Scopus

2-s2.0-85193597923