Trehalose During Two Stress Responses in Acanthamoeba: Differentiation Between Encystation and Pseudocyst Formation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F17%3A43895643" target="_blank" >RIV/60076658:12310/17:43895643 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/17:00480875 RIV/00216208:11110/17:10366198

Výsledek na webu

<a href="https://ac.els-cdn.com/S1434461017300779/1-s2.0-S1434461017300779-main.pdf?_tid=a5fe162a-f60e-11e7-a4ed-00000aacb361&acdnat=1515593032_79cbffc76bd685116d632a20412f3abc" target="_blank" >https://ac.els-cdn.com/S1434461017300779/1-s2.0-S1434461017300779-main.pdf?_tid=a5fe162a-f60e-11e7-a4ed-00000aacb361&acdnat=1515593032_79cbffc76bd685116d632a20412f3abc</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.protis.2017.09.001" target="_blank" >10.1016/j.protis.2017.09.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Trehalose During Two Stress Responses in Acanthamoeba: Differentiation Between Encystation and Pseudocyst Formation

Popis výsledku v původním jazyce

The non-reducing disaccharide trehalose can serve as a protectant against a range of environmental stressors, such as heat, cold, or dehydration, in both prokaryotes and eukaryotes, with the exception of vertebrates. Here, we analyzed trehalose metabolism in the facultatively parasitic organism Acanthamoeba castellanii, known to respond to unfavorable external conditions by forming two resistant stages: a cyst, produced in the case of chronic stress, and a pseudocyst, formed in reaction to acute stress. The possible role of trehalose in the resistant stages was investigated using a combination of bioinformatic, molecular biological and biochemical approaches. Genes for enzymes from a widespread trehalose-6-synthase-trehalose-6-phosphate phosphatase (TPS-TPP) pathway and a prokaryotic trehalose synthase (TreS) pathway were identified. The expression patterns of the genes during encystation and pseudocyst formation were analyzed and correlated with the time course of cellular trehalose content determined mass spectrometrically. The data clearly demonstrate fundamental differences between encystation and pseudocyst formation at the level of cellular metabolism.

Název v anglickém jazyce

Trehalose During Two Stress Responses in Acanthamoeba: Differentiation Between Encystation and Pseudocyst Formation

Popis výsledku anglicky

The non-reducing disaccharide trehalose can serve as a protectant against a range of environmental stressors, such as heat, cold, or dehydration, in both prokaryotes and eukaryotes, with the exception of vertebrates. Here, we analyzed trehalose metabolism in the facultatively parasitic organism Acanthamoeba castellanii, known to respond to unfavorable external conditions by forming two resistant stages: a cyst, produced in the case of chronic stress, and a pseudocyst, formed in reaction to acute stress. The possible role of trehalose in the resistant stages was investigated using a combination of bioinformatic, molecular biological and biochemical approaches. Genes for enzymes from a widespread trehalose-6-synthase-trehalose-6-phosphate phosphatase (TPS-TPP) pathway and a prokaryotic trehalose synthase (TreS) pathway were identified. The expression patterns of the genes during encystation and pseudocyst formation were analyzed and correlated with the time course of cellular trehalose content determined mass spectrometrically. The data clearly demonstrate fundamental differences between encystation and pseudocyst formation at the level of cellular metabolism.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA310%2F09%2F1120" target="_blank" >GA310/09/1120: Diferenciace volně žijících améb rodu Acanthamoeba a Balamuthia</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Protist

ISSN

1434-4610

e-ISSN

—

Svazek periodika

168

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

14

Strana od-do

649-662

Kód UT WoS článku

000416347800001

EID výsledku v databázi Scopus

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