DNA content in Acanthamoeba during two stress defense reactions: Encystation, pseudocyst formation and cell cycle

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903425" target="_blank" >RIV/60076658:12310/21:43903425 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/21:00548210 RIV/00216208:11110/21:10427329

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0932473920300754?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0932473920300754?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ejop.2020.125745" target="_blank" >10.1016/j.ejop.2020.125745</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DNA content in Acanthamoeba during two stress defense reactions: Encystation, pseudocyst formation and cell cycle

Popis výsledku v původním jazyce

During environmental stress, the vegetative cells of the facultative pathogenic amoeba Acanthamoeba castellanii reversibly differentiate into resistant dormant stages, namely, cysts or pseudocysts. The type of resistant stage depends on the nature and duration of the stressor. Cell differentiation is accompanied by changes in morphology and cellular metabolism. Moreover, cell differentiation is also expected to be closely linked to the regulation of the cell cycle and, thus, to cellular DNA content. While the existence of the resistant stages in A. castellanii is well known, there is no consensus regarding the relationship between differentiation and cell cycle progression. In the present work, we used flow cytometry analysis to explore the changes in the DNA content during Acanthamoeba encystation and pseudocyst formation. Our results strongly indicate that A. castellanii enters encystation from the G2 phase of the cell cycle. In contrast, differentiation into pseudocysts can begin in the G1 and G2 phases. In addition, we present a phylogenetic analysis and classification of the main cell cycle regulators, namely, cyclin-dependent kinases and cyclins that are found in the genome of A. castellanii. (C) 2020 Elsevier GmbH. All rights reserved.

Název v anglickém jazyce

DNA content in Acanthamoeba during two stress defense reactions: Encystation, pseudocyst formation and cell cycle

Popis výsledku anglicky

During environmental stress, the vegetative cells of the facultative pathogenic amoeba Acanthamoeba castellanii reversibly differentiate into resistant dormant stages, namely, cysts or pseudocysts. The type of resistant stage depends on the nature and duration of the stressor. Cell differentiation is accompanied by changes in morphology and cellular metabolism. Moreover, cell differentiation is also expected to be closely linked to the regulation of the cell cycle and, thus, to cellular DNA content. While the existence of the resistant stages in A. castellanii is well known, there is no consensus regarding the relationship between differentiation and cell cycle progression. In the present work, we used flow cytometry analysis to explore the changes in the DNA content during Acanthamoeba encystation and pseudocyst formation. Our results strongly indicate that A. castellanii enters encystation from the G2 phase of the cell cycle. In contrast, differentiation into pseudocysts can begin in the G1 and G2 phases. In addition, we present a phylogenetic analysis and classification of the main cell cycle regulators, namely, cyclin-dependent kinases and cyclins that are found in the genome of A. castellanii. (C) 2020 Elsevier GmbH. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Protistology

ISSN

0932-4739

e-ISSN

—

Svazek periodika

77

Číslo periodika v rámci svazku

FEB 2021

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

000615988500001

EID výsledku v databázi Scopus

2-s2.0-85096405645